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一株高效硝化菌的鉴定及其特性     被引量:5

Identification and characteristics of a high-efficient nitrification bacterium

文献类型:期刊文献

中文题名:一株高效硝化菌的鉴定及其特性

英文题名:Identification and characteristics of a high-efficient nitrification bacterium

作者:邓斌[1];郑佳佳[1];傅罗琴[1];张小平[1];梁权[1];沈文英[2];李卫芬[1]

机构:[1]浙江大学动物科学学院农业部动物营养与饲料重点实验室;[2]绍兴文理学院生命科学学院

年份:2013

卷号:7

期号:9

起止页码:3635

中文期刊名:环境工程学报

外文期刊名:Chinese Journal of Environmental Engineering

收录:CSTPCD、、北大核心2011、Scopus、北大核心、CSCD、CSCD2013_2014

基金:国家"973"重点基础研究发展规划项目(2009CB118705);公益性行业(农业)科研专项(201203083);国家"水体污染控制与治理"科技重大专项(2012ZX07101-012-03)

语种:中文

中文关键词:养殖水体;氨氮;降解;枯草芽孢杆菌

外文关键词:aquaculture water; ammonia-nitrogen; degradation; Bacillus subtilis

中文摘要:本研究通过测定菌株ABT01在不同初始氮浓度、pH、C/N、温度和溶氧条件下对氨氮的去除效果,获得该菌株的最佳应用条件。实验结果表明,当初始氨氮浓度低于40 mg/L时,该菌株的氨氮去除率高达85%以上。该菌株最适脱氨氮条件均为:pH 5.0-7.0、C/N=5、35℃、摇床转速150 r/min(溶解氧5.1 mg/L),氨氮去除率最高达96.8%。同时该菌株经16S rDNA测序、细胞壁脂肪酸组成等鉴定方法,确定ABT01为枯草芽孢杆菌(Bacillus subtilis)。研究表明,枯草芽孢杆菌ABT01具有较好的氨氮去除能力,对水产养殖水质调控有潜在的应用价值。

外文摘要:In order to optimize the growth condition for the strain ABT01,we evaluated the ammonium removal efficiencies with different initial ammonium concentrations,pH,C / N,temperature and DO.The observed ammonium removal rate was more than 85% in 24 hours at an initial ammonium concentration below 40mg / L.The result showed that the best conditions for ammonium removal were as follows: pH 5.0 - 7.0,C / N = 5,35℃ and 150 r / min(DO is about 5.1 mg / L).Under the conditions,the highest ammonium removal rate could reach up to 96.8%.Through 16 S rDNA sequencing and fatty acid composition analysis of cell wall,the strain was identified as Bacillus subtilis.The results indicated that ABT01 had a potential role in ammonium removal in aquaculture practice.

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