详细信息
正交试验法优选羊栖菜多糖酶法提取工艺 被引量:12
Study on extraction process of polysaccharide from Sargassum fusiforme by enzymatic treatment
文献类型:期刊文献
中文题名:正交试验法优选羊栖菜多糖酶法提取工艺
英文题名:Study on extraction process of polysaccharide from Sargassum fusiforme by enzymatic treatment
作者:张华芳[1];金京顺[1];谈荣梅[1];沈澄[1]
机构:[1]绍兴文理学院医学院药理教研室
年份:2006
卷号:31
期号:22
起止页码:1860
中文期刊名:中国中药杂志
外文期刊名:China Journal of Chinese Materia Medica
收录:MEDLINE(收录号:17285983)、CSTPCD、、北大核心2004、Scopus(收录号:2-s2.0-33947156244)、CSCD2011_2012、北大核心、CSCD、PubMed
基金:浙江省教育厅项目(20031130)
语种:中文
中文关键词:正交试验;羊栖菜;多糖;纤维素酶
外文关键词:orthogonal design ; Sargassum fusiforme; polysaccharide ; cellulase
中文摘要:目的:优选羊栖菜多糖酶法的最佳提取工艺。方法:采用正交试验法进行优选,以褐藻胶得率和羊栖菜粗多糖含量为考察指标,对影响羊栖菜多糖酶法提取工艺的因素进行了研究。结果:酶量和酶处理温度对羊栖菜粗多糖提取有较大影响。结论:羊栖菜多糖酶法最佳提取工艺为酶处理10 m in,酶量1.2万U.100 g-1,pH 4.5,45℃。
外文摘要:ObJective: To investigate the optimal extraction process of polysaccharides from S.fusiforme by enzymatic treatment. Method: The optimum extraction conditions were obtained by the experiment with the orthogonal design. The content of polysaccharities of S. fus/forme was determined by spectraphotometry. Result:The amount of enzyme and temperature significantly affected total polysaccharides of S. fus/forme. Conclusion: The optimum extraction conditions include the addition of 1.2 × 10^4 U · 100 g ^-1 enzyme into water at pH 4.5, and the subsequent treatment for 10 min while the temperature is maintained at 45 ℃.
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