文献类型：期刊文献

英文题名：HMGB in Mollusk Crassostrea ariakensis Gould: Structure, Pro-Inflammatory Cytokine Function Characterization and Anti-Infection Role of Its Antibody

作者：Xu, Ting[1];Yang, Shoubao[2];Xie, Jiasong[1];Ye, Shigen[1,3];Luo, Ming[1];Zhu, Zewen[1];Wu, Xinzhong[1]

机构：[1]Zhejiang Univ, Coll Anim Sci, Lab Marine Life Sci & Technol, Hangzhou 310003, Zhejiang, Peoples R China;[2]Shaoxing Univ, Sch Life Sci, Shaoxing, Zhejiang, Peoples R China;[3]Dalian Ocean Univ, Coll Life Sci & Biotechnol, Dalian, Liaoning, Peoples R China

年份：2012

卷号：7

期号：11

外文期刊名：PLOS ONE

收录：SCI-EXPANDED(收录号：WOS:000312104900088)、、WOS

基金：This work was supported by research grants from the Fisheries Technical Extension Center of Guangxi Province, China and Zhejiang Province government grants (2007). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文摘要：Background: Crassostrea ariakensis Gould is a representative bivalve species and an economically important oyster in China, but suffers severe mortalities in recent years that are caused by rickettsia-like organism (RLO). Prevention and control of this disease is a priority for the development of oyster aquaculture. It has been proven that mammalian HMGB (high mobility group box) can be released extracellularly and acts as an important pro-inflammatory cytokine and late mediator of inflammatory reactions. In vertebrates, HMGB's antibody (anti-HMGB) has been shown to confer significant protection against certain local and systemic inflammatory diseases. Therefore, we investigated the functions of Ca-HMGB (oyster HMGB) and anti-CaHMGB (Ca-HMGB's antibody) in oyster RLO/LPS (RLO or LPS)-induced disease or inflammation. Methodology/Principal Findings: Sequencing analysis revealed Ca-HMGB shares conserved structures with mammalians. Tissue-specific expression indicates that Ca-HMGB has higher relative expression in hemocytes. Significant continuous up-regulation of Ca-HMGB was detected when the hemocytes were stimulated with RLO/LPS. Recombinant Ca-HMGB protein significantly up-regulated the expression levels of some cytokines. Indirect immunofluorescence study revealed that Ca-HMGB localized both in the hemocyte nucleus and cytoplasm before RLO challenge, but mainly in the cytoplasm 12 h after challenge. Western blot analysis demonstrated Ca-HMGB was released extracellularly 4-12 h after RLO challenge. Anti-Ca-HMGB was added to the RLO/LPS-challenged hemocyte monolayer and real-time RT-PCR showed that administration of anti-CaHMGB dramatically reduced the rate of RLO/LPS-induced up-regulation of LITAF at 4-12 h after treatment. Flow cytometry analysis indicated that administration of anti-CaHMGB reduced RLO/LPS-induced hemocyte apoptosis and necrosis rates. Conclusions/Significance: Ca-HMGB can be released extracellularly and its subcellular localization varies when stimulated with RLO. Ca-HMGB is involved in oyster immune reactions and functions as a pro-inflammatory cytokine. Anti-CaHMGB can significantly suppress RLO/LPS-induced inflammatory responses and hemocyte necrosis and apoptosis, suggesting that Ca-HMGB is a potential target to prevent and control RLO/LPS-induced disease or inflammation.