文献类型：期刊文献

英文题名：Characterization and Expression of Ailuropoda melanoleuca Leptin (ob gene)

作者：Xu, Qinggang[1,2];Zhang, Xinming[1];Han, Ning[1];Shou, Jianxin[1,3];Hou, Rong[4];Zhang, Zhihe[4];Bian, Hongwu[1];Zhu, Muyuan[1]

机构：[1]Zhejiang Univ, Key Lab Cell & Gene Engn Zhejiang Prov, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China;[2]Jiangsu Univ, Inst Life Sci, Zhenjiang 212013, Peoples R China;[3]Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Peoples R China;[4]Key Lab Conservat Biol Endangered Wildlife, Chengdu 610081, Sichuan, Peoples R China

年份：2010

卷号：27

期号：1

起止页码：41

外文期刊名：ZOOLOGICAL SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000273209300007)、、WOS

基金：This work was supported by grants from the Research Fund for Giant Panda Breeding of Chengdu (05GGSF140). We thank Dr Yong Wang and Dr Chiyu Zhang (Jiangsu University, China) for their revision of our manuscript.

语种：英文

外文关键词：leptin; Ailuropoda melanoleuca; cloning; expression; characterization

外文摘要：Leptin, an adipocyte-derived hormone, plays important roles in metabolism and reproduction. In this article, we report the cloning, expression, and identification of the giant panda leptin (gLeptin) gene and its variants. The gLeptin cDNA was 504 bp long, encoding a precursor peptide of 167 amino acids including 21 residues of signal peptide. A short variant of gLeptin was 501 bp long, encoding a 166-aa peptide and also including a 21-aa signal peptide. Giant panda leptin was 99.4%, 94.6%, and 92.8% identical to that of black bear, dog, and cat, respectively, but was only 81.4% and 80.8% identical to that of human and rat. The cloned gLeptin gene was expressed in Escherichia coli, with expression confirmed by Western blotting and MALDI-TOF-TOF MS PMF. After purification, renaturation, and condensation, the gLeptin protein was injected into Kunming mice. The recombinant gLeptin significantly inhibited food intake by 41.8% and reduced body weight by 5.1% in the mice.