文献类型：期刊文献

英文题名：Molecular Cytogenetic Characterization of the Genome Organization of the 6-Banded Armadillo (Euphractus sexcinctus)

作者：Liu, Y.[2];Ye, J.[1,2];Fu, B.[3];Ng, B. L.[3];Wang, J.[2];Su, W.[2];Yang, F.[3];Nie, W.[2]

机构：[1]Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Zhejiang, Peoples R China;[2]Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming, Peoples R China;[3]Wellcome Trust Sanger Inst, Hinxton, England

年份：2011

卷号：132

期号：1-2

起止页码：31

外文期刊名：CYTOGENETIC AND GENOME RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000283866600006)、、WOS

基金：We are grateful to Dr. G. Dobigny for his critical review of the manuscript, and to Dr. Julio Cesar Pieczarka for providing the armadillo skin biopsies. This study is supported partly by the grant from the National Natural Science Foundation of China (No. 30770293). B.F., B.L.N. and F.Y. are supported by the Wellcome Trust (Grant No. WT077008).

语种：英文

外文关键词：6-Banded armadillo; Ancestral Xenarthra karyotype; Cloning; Cross-species chromosome painting; FISH mapping; Repetitive DNAs; Sequencing

外文摘要：Xenarthra, as the probable earliest offshoot of the placental tree, represents a key taxon for understanding mammalian phylogeny. To gain further insight into the chromosomal evolution and genome organization of the xenarthrans, we have established the first genome-wide comparative chromosome map between human and the 6-banded armadillo (Euphractus sexcinctus, 2n = 58), a basal species on the Xenarthra branch, by reciprocal cross-species chromosome painting. In total, 22 human autosomal paints revealed 41 homologous segments in the euchromatic genome of E. sexcinctus. Our results provide further support for the notion that the 2 human homologous segmental associations, i.e. HSA 2/8 and 7a/10p, could constitute the synapomorphies that unite the xenarthrans. Moreover, we propose that the putative ancestral Xenarthra karyotype closely resemble the 2n = 54 karyotype of the E. sexcinctus, consisting of the equivalents of HSA1p, 1q, 2a, 2b, 2c/8c, 3/21, 4a, 4b/8b, 5, 6a, 6b, 7a/10p, 7b/16p, 8a, 9, 10q, 11, 12a/22a, 12b/22b, 13, 14/15, 16q/19q, 17, 18, 19p, 20, and X. In addition, we have analysed the C-banding patterns of E. sexcinctus, and cloned, FISH-mapped and sequenced 7 novel repetitive DNA segments, providing further information on the complexity of genome architecture of E. sexcinctus. Copyright (C) 2010 S. Karger AG, Basel