文献类型：期刊文献

中文题名：靛玉红-3′-单肟对猕猴成纤维细胞增殖和凋亡及周期同步化的影响

英文题名：Effects of indirubin-3′-monoxime on cell proliferation,apoptosis and cycle synchronization in rhesus monkey fibroblasts

作者：杨纪峰[1];采克俊[2];金立方[3];高基民[1]

机构：[1]温州医学院生命科学学院;[2]湖州师范学院生命科学学院;[3]绍兴文理学院生命科学学院

年份：2011

卷号：39

期号：5

起止页码：8

中文期刊名：西北农林科技大学学报：自然科学版

收录：CSTPCD、、CSCD2011_2012、北大核心2008、北大核心、CSCD

基金：浙江省自然科学基金项目(Y2080996);温州医学院科研启动基金项目(QTJ07018)

语种：中文

中文关键词：靛玉红-3′-单肟;猕猴成纤维细胞;细胞增殖;细胞凋亡;周期同步化

外文关键词：indirubin-3′-monoxime; rhesus monkey fibroblast; proliferation; apoptosis; cycle synchronization

中文摘要：【目的】研究靛玉红-3′-单肟(Indirubin-3′-monoxime)对猕猴成纤维细胞增殖、凋亡和周期同步化的影响。【方法】用PI和FITC同时对细胞进行染色,用流式细胞仪检测荧光强度,对细胞内DNA和蛋白质的含量作相对定量,分析细胞在G0,G0+G1,S,G2+M期的分布比例;用BrdU标记法检测靛玉红-3′-单肟处理对细胞增殖的影响,用原位末端标记法(TUNEL)检测细胞凋亡的发生情况。【结果】(1)与处于对数增殖期(对照)的细胞相比,10μmol/L靛玉红-3′-单肟处理30 h后,G0与G0+G1期细胞比例分别由7.27%和84.26%增加为14.21%和94.22%,差异达显著水平。(2)与对数增殖期细胞(BrdU阳性率为80%)相比,靛玉红-3′-单肟处理30 h可显著地抑制细胞的增殖,在后续标记培养24 h期间只有8.15%的细胞呈BrdU阳性,如果是在去除药物的正常条件下标记培养24 h,大多数细胞(58.28%)又可进入增殖状态。(3)与正常培养对照组细胞凋亡率(1.12%)相比,靛玉红-3′-单肟处理组凋亡发生率为1.82%,虽有所增加但差异不显著。【结论】靛玉红-3′-单肟是一种可逆的细胞周期抑制剂,对猕猴成纤维细胞增殖的抑制作用是可逆的,对细胞凋亡发生率没有显著影响。

外文摘要：【Objective】 This study was to investigate the effects of indirubin-3′-monoxime on proliferation,apoptosis and cycle synchronization of rhesus monkey fibroblasts.【Method】 Cells were stained with PI and FITC,then were analyzed with flow cytometry to detect the distribution of cells in G0,G0＋G1,S,and G2＋M stages.Cell proliferation was analyzed with BrdU incorporation into new synthesized DNA synthesis.Apoptosis was detected with in situ TUNEL analysis.【Result】（1）Compared with cycling cells,proportions of G0 and G0＋G1 cells significantly increased from 7.27% and 84.26% to 14.21% and 94.22% in cultures treated with 10 μmol/L indirubin-3′-monoxime.（2）During 24 h BrdU labeling culture,cell proliferation was significantly inhibited and only 8.15% of indirubin-3′-monoxime treated cells were BrdU-positive in contrast to 80% of cycling control cultures.However,after removal of indirubin-3′-monoxime,58.28% cells reentered proliferation during 24 h labeling.（3）Apoptosis occurrence increased,but not significantly,in cultures treated with indirubin-3′-monoxime（1.82%） compared with normal control cultures（1.12%）.【Conclusion】 Indirubin-3′-monoxime is a kind of reversible cycle inhibitor for rhesus monkey fibroblasts,which can inhibit cell proliferation in a reversible way and does not induce apoptosis.