文献类型：期刊文献

中文题名：HPLC法测定阿达帕林脂质体中药物含量及包封率

英文题名：HPLC determination of content and entrapment efficiency of adapalene liposomes

作者：刘利萍[1];胡六江[1];周晓芬[1]

机构：[1]绍兴文理学院化学化工学院药学系

年份：2007

卷号：27

期号：9

起止页码：1462

中文期刊名：药物分析杂志

外文期刊名：Chinese Journal of Pharmaceutical Analysis

收录：CSTPCD、、北大核心2004、CSCD2011_2012、北大核心、CSCD

语种：中文

中文关键词：阿达帕林脂质体;高效液相色谱法;含量测定;包封率

外文关键词：adapalene liposomes ; HPLC ; content ; entrapment efficiency ene was 95.55% - 100. 3% Conclusion： The method is

中文摘要：目的:建立阿达帕林脂质体中药物含量和包封率的 HPLC 测定方法。方法:采用 Hypersil ODS 2柱(250 mm×4.6 mm,5μm),以20 mmol·L^(-1)醋酸铵溶液(用氨水调 pH 至7.4)-甲醇(10:90)为流动相,流速1.0 mL·min^(-1),柱温30℃,检测波长270 nm。采用4℃、10000 r·min^(-1)离心30 min 分离脂质体和游离药物,测定包封率。结果:在本色谱条件下辅料和溶剂不干扰药物测定,阿达帕林进样浓度在6.12～36.72μg·mL^(-1)范围内与峰面积呈现良好线性关系(r=0.9996),回收率在98.64%～103.0%之间(RSD 为0.59%～0.90%),日内及日间精密度的 RSD 均小于1%(n=5)。高速离心分离法对游离药物的回收率为95.55%～100.3%(RSD 为1.3%～1.4%)。结论:采用高速离心-HPLC 法测定阿达帕林脂质体中药物含量和包封率简便快速,结果可靠。

外文摘要：Objective：To establish an HPLC method for the determination of content and entrapment efficiency of adapalene liposomes. Method ：A Hypersil ODS 2 column（250 mm × 4. 6 mm,5 μm）was used with 20 mmol · L^-1 ammonium acetate solution（adjusted to pH 7.4 with ammonia solution） -methanol（ 10： 90）as the mobile phase at a flow rate of 1.0 mL · min^ -1. The UV detection was set at 270 nm. The column temperature was 30 ℃. Entrapment efficiency was determined after the separation of the unentrapped drug by centrifugation at 10000 r · min^-1 for 30 min at 4 ℃. Results：h was found that the excipients in the liposome did not interfere with the assay. A good linear relationship was found between peak area and the concentration of adapalene in the range of 6. 12 - 36. 72 μg · mL^-1 （r =0. 9996） ,the sample recoveries were 98. 64% -103.0%with RSD of 0. 59% -0. 90% ,RSDs of the intra - and inter - day precision were lower than 1% （ n = 5 ）. The recovery of free adapal with RSD of 1.3% - 1.4% from liposome suspensions by high - speed centrifugation. quick, accurate, feasible and applicable.