文献类型：期刊文献

英文题名：Assessment of pulmonary toxicity of potential antioxidant drug PEGylated nanoceria after intratracheal instillation in rats

作者：Qian, Qinqing[1];Zhang, Yun[1];Chen, Yuan[1];Ye, Chenqiao[1];Feng, Qiang[1];Tu, Jinqing[1];Lu, Zhenbo[1];Xu, Yilan[1];Ran, Na[1];Xing, Guiying[1];Yu, Zhangsen[1]

机构：[1]Shaoxing Univ, Sch Med, Med Sci Res Ctr, Lab Nanomed, Shaoxing 312000, Peoples R China

年份：2021

卷号：41

期号：6

起止页码：941

外文期刊名：JOURNAL OF APPLIED TOXICOLOGY

收录：SCI-EXPANDED(收录号：WOS:000580980100001)、、Scopus(收录号：2-s2.0-85093509058)、WOS

基金：Science and Technology Plan in Shaoxing City, Grant/Award Number: 2018C10011; National College Students Innovation and Entrepreneurship Training Program, China, Grant/Award Number: 201910349039X; Scientific Research Start-up Project of Shaoxing University, Grant/Award Number: 20185024; University Laboratory Work Research Project of Zhejiang Province, Grant/Award Number: YB201722; Educational Commission of Zhejiang Province, China, Grant/Award Number: Y201839726; College Students' Science and Technology Innovation Project of Zhejiang Province, Grant/Award Number: 2019R432024

语种：英文

外文关键词：autophagy; endoplasmic reticulum stress; intratracheal instillation; oxidative stress; PEGylated nanoceria; pulmonary toxicity

外文摘要：Cerium oxide (CeO2) nanoparticles have unique redox properties and exert excellent antioxidant effects in the biological environment. In recent years, many researchers have focused on the CeO(2)nanoparticles as an effective antioxidant drug in the prevention and treatment of various diseases. However, the toxicity of CeO(2)nanoparticlesin vivoremains controversial and still needs intensive research. Therefore, the objective of this study is to investigate the pulmonary and systemic toxicity in rats after 14 days of exposure to the PEGylated CeO(2)nanoparticles (abbreviated as CNPs; exposure dose of 2, 10, or 20 mg/kg) through a single intratracheal instillation (IT). We assessed the indicators of lung injury and the pathological damage degree of lung tissue. The bronchoalveolar lavage fluid (BALF) analysis and lung histopathology revealed the occurrence of slight pulmonary inflammation in the 20-mg/kg experimental group rats. However, the inflammation factors in the lung tissue of every group rats did not significantly increase, and the levels of superoxide dismutase (SOD) and glutathione (GSH) in lung tissue homogenate rose considerably in the experimental groups. Collectively, these results indicated that pulmonary exposure by the high dose of CNPs could induce mild pulmonary inflammation but did not cause severe systemic toxicity. Moreover, we speculate that the mechanism of pulmonary toxicity of CNPs in rats was due to the autophagic death of healthy lung epithelial cells mediated by endoplasmic reticulum stress. Our results implicate that CNPs can be safely used as an antioxidant drug for the oxidative stress pulmonary diseases.