文献类型：期刊文献

中文题名：竹叶多糖的组分及抗氧化活性分析

英文题名：Analysis of composition and antioxidant activities of polysaccharide extracted from Moso Bamboo-Leaf

作者：殷军[1,2,3];葛青[2,3];毛建卫[2,3];方晟[3,4]

机构：[1]浙江理工大学理学院;[2]浙江科技学院生物与化学工程学院;[3]浙江省农产品化学与生物加工技术重点实验室;[4]绍兴文理学院元培学院,生命科学系

年份：2013

卷号：34

期号：2

起止页码：100

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD、、北大核心2011、北大核心、CSCD、CSCD2013_2014

基金：国家"十二五"科技支撑项目(2011BAD23B02);浙江省重大科技专项农业项目(2008C12036);浙江省首批重点创新团队项目(2009R50028);浙江省重点实验室开放基金(2010KF0506);浙江科技学院科研启动基金资助项目(F501103906)

语种：中文

中文关键词：竹叶;多糖;组成;抗氧化活性

外文关键词：Bamboo-Leaf; polysaccharides; composition; antioxidant activities

中文摘要：竹叶粉碎后经过高温水提醇沉得竹叶多糖粗品。用DEAE琼脂糖凝胶FF和丙烯葡聚糖凝胶S-100分离纯化出两种均一多糖BLP30-1、BLP30-2,并对这二种多糖进行组分分析。气相分析结果表明,两者都由岩藻糖、木糖、甘露糖、葡萄糖、半乳糖五种糖组成。采用尺寸排除色谱和激光光散射联用仪(SEC-LLS)测定分子量分别为2.9×104、2.1×104u。对两种多糖进行抗氧化活性研究表明,其对DPPH自由基、超氧阴离子自由基、羟基自由基均有较高的抑制活性。

外文摘要：Crude polysaccharide was extracted by hot water and precipitated by ethanol. Two purified polysaccharides were obtained by DEAE Sepharose FF and Sephcryl S -100,components of BLP30 -1 and BLP30 -2 were studied by GC method. The result showed that they were all composed of fucose,xylose, mannose,glucose,galactose. The molecular weight of the polysaccharides was obtained by SEC -LLS and their molecular weight were 2.9×10 4 u and 2.1×10 4 u,respectively. Available data obtained with in vitro models suggested that they showed significant inhibitory effects on DPPH radical,hydroxyl radical,superoxide radical.