文献类型：期刊文献

英文题名：Antigenic characteristics of the complete and truncated capsid protein VP1 of enterovirus 71

作者：Zhang, Jianhua[1,3];Dong, Min[1];Jiang, Bingfu[1];Dai, Xing[2];Meng, Jihong[1]

机构：[1]Southeast Univ, Sch Med, Dept Microbiol & Immunol, Nanjing 210009, Jiangsu, Peoples R China;[2]Southeast Univ, Sch Med, Affiliated Zhongda Hosp, Dept Dermatol, Nanjing 210009, Jiangsu, Peoples R China;[3]Shaoxing Univ, Sch Med, Dept Microbiol & Immunol, Shaoxing 312000, Zhejiang, Peoples R China

年份：2012

卷号：167

期号：2

起止页码：337

外文期刊名：VIRUS RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000306889100025)、、Scopus(收录号：2-s2.0-84863860557)、WOS

语种：英文

外文关键词：Enterovirus; Viral protein; Epitope mapping; Neutralization; Monoclonal antibody

外文摘要：The complete VP1 protein of enterovirus 71 (EV71) and a series of truncations were expressed in Escherichia coli and their antigenic characteristics were studied. Ilmmunoblot analysis showed the major immunoreactive region of the VP1 protein was located in the N-terminal portion at position of amino acid (aa) 1-100. The complete VP1 possessed strong cross-reactivity with antisera against coxsackievirus A16 (CA16) and echovirus 6 (Echo6), while the truncated fragment at position 1-100 aa only had weak cross-reactivity. Moreover, an EV71-specific linear epitope at position 94-105 aa was identified using two EV71-specific mAbs (2B9 and 587) with indirect ELISA, but could not be recognized by antibodies against EV71 virus particles. The complete and all of truncated VP1 proteins except His-VP1(202_297) and GST-VP1(202-248) failed to elicit a significant neutralizing antibody response in mice. His-VP1(202-297) and GST-VP1(202-248) containing neutralizing epitope(s) could be recognized only by anti-EV71 mouse sera but not rabbit or human sera. These findings may contribute to a further understanding of antigenic characteristics of the capsid protein VP1 and may be helpful to the development of diagnostic reagents and vaccines. (c) 2012 Elsevier B.V. All rights reserved.