文献类型：期刊文献

英文题名：Monodisperse CaCO3-loaded gelatin microspheres for reversing lactic acid-induced chemotherapy resistance during TACE treatment

作者：Chen, Minjiang[1,2,4];Guo, Xiaoju[1,3];Shen, Lin[1];Ding, Jiayi[1];Yu, Junchao[1];Chen, Xiaoxiao[1,2,4];Wu, Fazong[4];Tu, Jianfei[1,2,3,4];Zhao, Zhongwei[1,2,3,4];Nakajima, Mitsutoshi[5];Song, Jingjing[1,2,4];Shu, Gaofeng[1,2,4];Ji, Jiansong[1,2,3,4]

机构：[1]Wenzhou Med Univ, Inst Imaging Diag & Minimally Invas Intervent Res, Key Lab Imaging Diag & Minimally Invas Intervent R, Affiliated Hosp 5, Lishui 323000, Peoples R China;[2]Lishui Univ, Affiliated Cent Hosp, Sch Med, Clin Coll, Lishui 323000, Peoples R China;[3]Shaoxing Univ, Sch Med, Shaoxing 312000, Peoples R China;[4]Zhejiang Univ, Sch Med, Dept radiol, Lishui Hosp, Lishui 323000, Peoples R China;[5]Univ Tsukuba, Fac Life & Environm Sci, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058572, Japan

年份：2023

卷号：231

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:000974610800001)、、EI(收录号：20242816666704)、Scopus(收录号：2-s2.0-85146315239)、WOS

基金：This work was supported by the National Key Research and Development projects intergovernmental cooperation in science and technology of China (2018YFE0126900) , Zhejiang Provincial Natural Science Foundation (LQ21H180003) , National Natural Science Foundation of China (81901848, 82072025, 81901852 and 82072026) , Key R & D Program of Lishui City (2021ZDYF12) , and Medical Health Science and Technology Project of Zhejiang Provincial Health Commission (2022RC088) .

语种：英文

外文关键词：Transarterial chemoembolization; Monodisperse microspheres; Microfluidic technique; Lactic acid; Calcium carbonate nanoparticles

外文摘要：Transarterial chemoembolization (TACE) is an important approach for the treatment of unresectable hepato-cellular carcinoma (HCC). However, the lactic acid-induced acidic tumor microenvironment (TME) may reduce the therapeutic outcome of TACE. Herein, monodispersed gelatin microspheres loaded with calcium carbonate nanoparticles (CaNPs@Gel-MS) as novel embolic agents were prepared by a simplified microfluidic device. It was found that the particle size and homogeneity of as-prepared CaNPs@Gel-MS were strongly dependent on the flow rates of continuous and dispersed phases, and the inner diameter of syringe needle. The introduction of CaNPs provided the gelatin microspheres with an enhanced ability to encapsulate the chemotherapeutic drug of DOX, as well as a pH-responsive sustained drug release behavior. In vitro results revealed that CaNPs@Gel-MS could largely increase the cellular uptake and chemotoxicity of DOX by neutralizing the lactic acid in the culture medium. In addition, CaNPs@Gel-MS exhibited an excellent and persistent embolic efficiency in a rabbit renal model. Finally, we found that TACE treatment with DOX-loaded CaNPs@Gel-MS (DOX/CaNPs@Gel-MS) had a much stronger ability to inhibit tumor growth than the DOX-loaded gelatin microspheres without CaNPs (DOX@Gel-MS). Overall, CaNPs@Gel-MS could be a promising embolic microsphere that can significantly improve anti-HCC ability by reversing lactic acid-induced chemotherapy resistance during TACE treatment.