文献类型：期刊文献

英文题名：PROTECTIVE EFFECT OF GRAPE SEED PROCYANIDIN EXTRACT AGAINST FLUORIDE-INDUCED HEPATIC INJURY IN RATS

作者：Wang, Er Hui[1];Ping, Guan Fang[2];Li, Hui[1];Sun, Yi Qin[1]

机构：[1]Shaoxing Univ, Med Coll, Dept Nursing, Shaoxing 312000, Zhejiang, Peoples R China;[2]Xinxiang Med Univ, Affiliated Hosp 1, Dept Pharm, Weihui 453100, Peoples R China

年份：2023

卷号：56

期号：4

起止页码：460

外文期刊名：FLUORIDE

收录：SCI-EXPANDED(收录号：WOS:001134373500004)、、Scopus(收录号：2-s2.0-85172482636)、WOS

基金：ACKNOWLEDGMENTS The authors thank Grants from the National Natural Science Foundation of China (Grant No.81703230) for financially support.

语种：英文

外文关键词：Grape seed procyanidin extract; Sodium fluoride; Oxidative stress; Hepatic apoptosis

外文摘要：This study aimed to determine the effects of grape seed procyanidin extract (GSPE) in alleviating sodium fluoride (NaF)-induced hepatic injury. Rats were randomly divided into four groups: control, NaF, NaF+GSPE, and NaF+Vc (vitamin C). After 28 days of intervention, key liver function-related biochemical parameters, oxidative stress markers, and histopathological changes in the hepatic tissue were analyzed. The oxidative stress was closely related to hepatic cell apoptosis as measured using the TUNEL method. In addition, the levels of apoptosis-related proteins Bax and Bcl-2 were determined by western blot. Compared with the control group, the NaF group showed reduced levels of antioxidant enzymes GSH, CAT, and SOD, with concomitantly increased MDA content, apoptosis, and histopathological damage in the liver tissues. However, GSPE administration significantly attenuated the oxidative stress and apoptosis in hepatic tissue and recovered histopathological damage. In addition, GSPE supplementation up-regulated anti-apoptotic Bcl-2 level and decreased pro-apoptotic Bax level. In conclusion, our results suggest that GSPE can exert antioxidant and anti-apoptotic protective effects against fluoride-induced hepatic injury in a rat model.