文献类型：期刊文献

英文题名：Excessive levels of nitric oxide in rat model of Parkinson's disease induced by rotenone

作者：Xiong, Zhong-Kui[1,2,3];Lang, Juan[4];Xu, Gang[5];Li, Hai-Yu[6];Zhang, Yun[7];Wang, Lei[3];Su, Yao[3];Sun, Ai-Jing[8]

机构：[1]Shaoxing Second Hosp, Dept Radiotherapy, Shaoxing 312000, Zhejiang, Peoples R China;[2]Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Radiotherapy, Shaoxing 312000, Zhejiang, Peoples R China;[3]Shaoxing Univ, Dept Clin Med, Sch Med, Shaoxing 312099, Zhejiang, Peoples R China;[4]Zhejiang Univ, Shaoxing Peoples Hosp, Med Res Ctr, Shaoxing 312000, Zhejiang, Peoples R China;[5]Jiangsu Univ, Affiliated Peoples Hosp, Dept Radiotherapy, Zhenjiang 212002, Jiangsu, Peoples R China;[6]Shaoxing Univ, Sch Med, Dept Lab Med, Shaoxing 312099, Zhejiang, Peoples R China;[7]Shaoxing Univ, Sch Med, Dept Basic Med, Shaoxing 312099, Zhejiang, Peoples R China;[8]Zhejiang Univ, Shaoxing Peoples Hosp, Dept Pathol, Shaoxing 312000, Zhejiang, Peoples R China

年份：2015

卷号：9

期号：2

起止页码：553

外文期刊名：EXPERIMENTAL AND THERAPEUTIC MEDICINE

收录：SCI-EXPANDED(收录号：WOS:000349506900047)、、Scopus(收录号：2-s2.0-84918773926)、WOS

基金：This study was supported by grants from the Natural Science Foundation of Zhejiang Province (nos. Y2100035 and Y2111046), the Key Medical Discipline Construction Project of Zhejiang Province (no. GJSX-010-004), the Science and Technology Development Project of Zhejiang Province (no. 2010C33116), the Science and Technology Development Project of Shaoxing, Zhejiang Province (no. 2010A23021), the Program from Zhejiang Provincial Administration of Traditional Chinese Medicine (nos. 2011ZA108 and 2012ZB162) and the Program from the Department of Education of Zhejiang Province (no. Y201018615).

语种：英文

外文关键词：nitric oxide; rotenone; Parkinson's disease

外文摘要：Systemic rotenone models of Parkinson's disease (PD) are highly reproducible and may provide evidence on the pathogenesis of PD. In the present study, male Sprague-Dawley rats (1-year-old) were subcutaneously administered with rotenone (1.5 mg/kg/day) for six days and observed for the following three weeks. Compared with the control rats, a significant decrease was observed in the body weight and a marked increase was observed in the areas under the behavioral scoring curves in the rotenone-treated rats. Immunohistochemical staining revealed that the abundance of nigral tyrosine hydroxylase (TH)-positive neurons was markedly reduced following rotenone treatment. ELISA and neurochemical assays demonstrated a significant increase in the levels of nitric oxide (NO) and NO synthase, whereas a marked decrease was observed in the thiol levels in the brains of the rotenone-treated rats. Thus, subacute rotenone treatment was found to induce behavioral deficits and the loss of nigral TH-positive neurons which may be associated with the excessive levels of NO in the rat brains.