文献类型：期刊文献

英文题名：ADAPTOR PROTEIN-1 complex-mediated post-Golgi trafficking is critical for pollen wall development in Arabidopsis

作者：Xu, Mei[1];Yan, Xu[1];Wang, Yutong[1];Liu, Chan[1];Yang, Qian[1];Tian, Dan[1];Bednarek, Sebastian Y.[2];Pan, Jianwei[1];Wang, Chao[1,3]

机构：[1]Lanzhou Univ, Sch Life Sci, Key Lab Cell Act & Stress Adaptat, Minist Educ, Lanzhou 730000, Peoples R China;[2]Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA;[3]Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Zhejiang, Peoples R China

年份：0

外文期刊名：NEW PHYTOLOGIST

收录：SCI-EXPANDED(收录号：WOS:000798528400001)、、Scopus(收录号：2-s2.0-85130272095)、WOS

基金：This work was supported by grants to JP from the National Natural Science Foundation of China (nos. 91754104, 31820103008 and 31670283) and to CW from the Youth Program of National Natural Science Foundation of China (no. 31801193). We are grateful to Zhongnan Yang (College of Life and Environment Sciences, Shanghai Normal University), Cheng Zhang (College of Life and Environment Sciences, Shanghai Normal University) and Yan Zhang (College of Life Sciences, Shandong Agricultural University) for generously sharing published materials and comments and to the Arabidopsis Biological Resource Center for seed stocks. We thank Liang Peng, Yang Zhao, Liping Guan, Yahu Gao, Xia Deng and Haiyan Li (Core Facility for Life Science Research, Lanzhou University) for their technical assistance as well as Jia Li and Xiaoping Gou (School of Life Sciences, Lanzhou University) for the CRISPR/Cas9, BiFC and Y2H vectors.

语种：英文

外文关键词：AP-1; Arabidopsis thaliana; microspore; pollen wall; post-Golgi trafficking; primexine; tapetum

外文摘要：Primexine deposition is essential for the formation of pollen wall patterns and is precisely regulated by the tapetum and microspores. While tapetum- and/or microspore-localized proteins are required for primexine biosynthesis, how their trafficking is established and controlled is poorly understood. In Arabidopsis thaliana, AP1 sigma 1 and AP1 sigma 2, two genes encoding the sigma subunit of the trans-Golgi network/early endosome (TGN/EE)-localized ADAPTOR PROTEIN-1 complex (AP-1), are partially redundant for plant viability, and the loss of AP1 sigma 1 function reduces male fertility due to defective primexine formation. Here, we investigated the role of AP-1 in pollen wall formation. The deposition of Acyl-CoA SYNTHETASE5 (ACOS5) and type III LIPID TRANSFER PROTEINs (LTPs) secreted from the anther tapetum, which are involved in exine formation, were impaired in ap1 sigma 1 mutants. In addition, the microspore plasma membrane (PM) protein RUPTURED POLLEN GRAIN1 (RPG1), which regulates primexine deposition, accumulated abnormally at the TGN/EE in ap1 sigma 1 mutants. We show that AP-1 mu recognizes the YXX phi motif of RPG1, thereby regulating its PM abundance through endocytic trafficking, and that loss of AP1 sigma 1 decreases the levels of other AP-1 subunits at the TGN/EE. Our observations show that AP-1-mediated post-Golgi trafficking plays a vital role in pollen wall development by regulating protein transport in tapetal cells and microspores.