文献类型：会议论文

英文题名：Microbial transformation of saponins in Dioscorea zingiberensis for diosgenin production with Trichoderma reesei

作者：Yu, Li Dan[1]; Zheng, Tian Xiang[1]; Zhu, Yu Ling[1]

机构：[1] College of Life Sciences, Shaoxing University, Shaoxing 312000, China

会议论文集：Advances in Applied Science, Engineering and Technology

会议日期：May 19, 2013 - May 21, 2013

会议地点：Qingdao, China

语种：英文

外文关键词：Metabolites

外文摘要：Microbial transformation of plant tubers for diosgenin production is an attractive clean method in industry. The purpose of this study was to evaluate the quality of the product obtained this way. Changes of saponins during the hydrolysis of Dioscorea zingiberensis tubers by Trichoderma reesei were investigated. Four main saponins were isolated from the plant herbs. Sugar chains, which connected to the C-3 hydroxyl group in saponins, were cleaved stepwisely by Trichoderma reesei from the terminal sugar until the aglycone was released. Diosgenin-3-O-β-D-glucose was a byproduct in this process. While, in the petrol extract of the microbial hydrolyzate, only diosgenin was detected. Melting point, IR spectra and 13C NMR spectroscopy were used to estimate the quality of the product. Diosgenin obtained from bioconversion showed similar characteristic to that from acid hydrolyzation, confirming microbial transformation was an effective method for diosgenin production. ? (2013) Trans Tech Publications, Switzerland.