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香榧假种皮提取物对潜叶跳甲的杀虫活性初步研究     被引量:4

Study on Insecticidal Activities of Extract from the Aril of Torreya grandis against Argopistes tsekooni Chen

文献类型:期刊文献

中文题名:香榧假种皮提取物对潜叶跳甲的杀虫活性初步研究

英文题名:Study on Insecticidal Activities of Extract from the Aril of Torreya grandis against Argopistes tsekooni Chen

作者:单麟珉[1];孙小红[1]

机构:[1]绍兴文理学院元培学院生命科学系

年份:2013

卷号:52

期号:9

起止页码:2060

中文期刊名:湖北农业科学

外文期刊名:Hubei Agricultural Sciences

收录:CSTPCD、、北大核心2011、北大核心

基金:绍兴市2011年科技计划项目(2011A22009);2011年度绍兴市大学生科技创新项目(绍市教高〔2012〕15号)

语种:中文

中文关键词:香榧(Torreya;grandis;cv;Merrilli)假种皮;提取物;女贞潜叶跳甲(Argopistes;tsekooni;Chen);杀虫活性

外文关键词:Torreya grandis cv. Merrillii aril; extract; A rgopistes tsekooni ; insecticidal activity

中文摘要:为研究香榧(Torreya grandis cv.Merrilli)假种皮的杀虫活性,采用液-液萃取分离法对香榧假种皮乙醇提取浸膏进行萃取分离(石油醚、氯仿、乙酸乙酯、正丁醇),并采用水蒸气蒸馏法提取挥发油,采用叶碟浸渍法分别测定5种提取物对潜叶跳甲(Argopistes tsekooni)的杀虫活性。结果表明,5种处理液均有较好的杀虫活性,且浓度越高杀虫活性越强。石油醚提取物的杀虫活性最好,浓度达到200 mg/mL以上时处理96 h的校正死亡率达到100%,其次为氯仿提取物。毒力测定结果表明,石油醚提取物LD50最小,为32.82 mg/mL。因此,香榧假种皮提取物表现出较好的抑虫活性,具有进一步研究开发的价值。

外文摘要:In order to understand the insecticidal activities of aril of Torreya grandis cv. Merrillii, ethanolic extract of aril of T. grandis was separated by different solvents (petroleum ether, chloroform, ethyl acetate, n-butyl alcohol) by liquid-liquid partition method; and the volatile oil was extracted by steam distillation method. The insecticidal activities of the 5 extracts against Argopistes tsekooni Chen were tested by leaf butterfly impregnation method. The results showed that the 5 extracts had good insecticidal activity which increased with the increase of the concentration of extracts. Petroleum ether extracts had the highest insecticidal efficient; the corrected mortalities in 96h was 100% when the concentration was 200mg/mL. The insecti- cidal activity of chloroform was the next. Toxicity test results showed that the LD50 of the petroleum ether extracts of T. gran- dis aril against A. tsekooni was 32.82 mg/mL. Therefore, the crude extracts of T. grandis aril had good insecticidal activity, and had excellent value for further research and development.

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