文献类型：期刊文献

中文题名：褪黑素促进镉胁迫下番茄种子萌发的调控机理

英文题名：Regulatory Mechanism of Melatonin on Tomato Seed Germination Under Cd2+Stress

作者：蔡淑钰[1];刘建新[1];王国夫[1];吴丽元[1];宋江平[1]

机构：[1]绍兴文理学院元培学院,绍兴312000

年份：2023

卷号：58

期号：5

起止页码：720

中文期刊名：植物学报

外文期刊名：Chinese Bulletin of Botany

收录：CSTPCD、、CSCD2023_2024、北大核心、CSCD、北大核心2020

基金：国家自然科学基金(No.31902095)。

语种：中文

中文关键词：褪黑素;镉胁迫;番茄;种子萌发

外文关键词：melatonin;cadmium stress;tomato;seed germination

中文摘要：土壤镉(Cd2+)污染严重制约设施蔬菜的产量和品质。褪黑素(MT)可以增强植株对多种逆境的抗性,然而Cd2+胁迫下MT调控番茄(Solanum lycopersicum)种子萌发的下游信号尚不清楚。该研究以番茄野生型Alisa Craig种子为材料,探究不同浓度Cd2+处理及外源MT对番茄种子萌发的调控效应。结果表明,0.5 mmol·L^(-1)以上的Cd2+处理显著抑制番茄种子的萌发和幼苗生长。用0.15 mmol·L^(-1)MT浸种可降低种子萌发后地下部和地上部的Cd2+含量,有效缓解Cd2+胁迫对番茄种子萌发和幼苗生长的抑制作用。MT上调了番茄胚根中植物螯合肽和转运蛋白基因(PCS、NRAMP1、ABCC3、HMA3与ABCG5)的表达,促进Cd2+的跨膜转运以及液泡隔离。此外,MT减轻了Cd2+诱导的氧化损伤,除褪黑素自身的抗氧化作用外,也与抗氧化酶(CAT、APX与ALDH)活性的增强相关。进一步分析表明,MT显著下调了Cd2+胁迫下脱落酸(ABA)合成基因(NCED1和NCED2)的表达,并上调了ABA分解基因ABA8ox1的表达,降低ABA含量,从而有效调节赤霉素/脱落酸(GA/ABA)的比值,促进Cd2+胁迫下番茄种子的萌发。

外文摘要：Soil cadmium(Cd)pollution seriously restricts the yield and quality of facility vegetables.Melatonin(MT)can enhance the resistance to various stresses in plants.However,the downstream signals,which regulated by MT during tomato seed germination under Cd2+stress remains unclear.The effects of Cd2+stress and exogenous MT on seed germination were investigated using tomato(Solanum lycopersicum)wild-type Alisa Craig seeds.The results showed that the germination of tomato seeds and seedling growth were significantly inhibited by Cd2+treatment with more than 0.5 mmol·L^(-1).Exogenous MT(0.15 mmol·L^(-1))reduced the content of Cd2+in the underground and above-ground tissues of seedlings,effectively alleviating the inhibitory effect of Cd2+stress on tomato seed germination and seedling length.The expression of phytochelatin and transporters-related genes(PCS,NRAMP1,ABCC3,HMA3,and ABCG5)in tomato radicles were significantly increased by MT under Cd2+stress,showing the positive regulation of MT in transmembrane transport and vacuolar sequestration of Cd2+.In addition,MT alleviated the oxidative damage induced by Cd2+stress,which was related to the enhanced activities of CAT,APX,and ALDH enzymes apart from its own scavenging ability.Furthermore,MT significantly down-regulated the expressions of abscisic acid(ABA)synthesis genes(NCED1 and NCED2)under Cd2+stress,and up-regulated the expression of ABA decomposition gene ABA8ox1,leading to the decrease of ABA content,which effectively regulated the GA/ABA ratio and promoted the germination of tomato seeds under Cd2+stress.