文献类型：期刊文献

中文题名：桃形李果实丝衣霉菌的分离、鉴定、生物学特性及其防治

英文题名：Isolation, Identification, Physicochemical Parameters and Antibacterial Characteristics of Byssochlamys sp. from Shengzhou Nane(Prunus salicina var. taoxingli) Fruit

作者：莫亿伟[1];林海雁[1];郑梦琪[1];王海[1];杨国[1];王玮[2]

机构：[1]绍兴文理学院生命科学学院,绍兴312000;[2]绍兴文理学院元培学院,绍兴312000

年份：2019

卷号：27

期号：12

起止页码：2227

中文期刊名：农业生物技术学报

外文期刊名：Journal of Agricultural Biotechnology

收录：CSTPCD、、北大核心2017、CSCD2019_2020、北大核心、CSCD

基金：绍兴公益性技术应用研究计划项目(No.2017B70059,No.2018C20008);浙江省科技厅公益项目(No.LGN20C150004);2013年嵊州市本级科技项目(No.64)

语种：中文

中文关键词：桃形李;丝衣霉菌;生物学特性;NaHCO3;寡聚糖

外文关键词：Shengzhou Nane;Byssochlamys sp.;Biological characteristics;NaHCO3;Chitosan oligosaccharide

中文摘要：桃形李(Prunus salicina var.taoxingli)成熟于7月中旬高温多雨的季节,果实采收前后易受多种霉菌侵染而导致大量落果,也难以保鲜。为了鉴定桃形李致病菌种类和降低霉菌发生,本研究在鉴定致病菌的基础上,对其可能的宿主进行检测,并探讨光照、温度、pH值及不同C源和N源对菌落生长的影响;利用NaHCO3结合壳寡糖(chitosan oligosaccharide,COS)涂膜处理进行防效试验。结果表明,致病菌为丝衣霉菌(Byssochlamys sp.)B-TXL-02,该菌株对梨(Pyrus spp.)、苹果(Malus domestica)、桃(Prunus persica)、樱桃(Prunus avium)、葡萄(Vitis vinifera)、杏(Prunus armeniaca)、芒果(Mangifera indica)和香蕉(Musa spp.)等水果均可致病,说明其宿主具广谱性。连续光照可显著抑制此致病菌的菌落生长。该菌最适生长条件为:温度30℃、pH 5,最适C源为D-果糖和葡萄糖;最适宜N源为牛肉浸出膏和酵母浸出膏。添加NaHCO3和壳寡糖在马铃薯葡萄糖琼脂培养基(potato dextrose agar,PDA)上培养的前6 d和前12 d能显著抑制菌落生长,这两种药剂均可显著抑制孢子萌发和菌丝生长,且经0.3%NaHCO3+1.6%壳寡糖涂膜复合处理可显著降低果实腐烂率。研究结果为桃形李果实采后丝衣霉菌的防治提供一种实用方法。

外文摘要：Shengzhou Nane(Prunus salicina var.taoxingli)usually ripe in mid-July,high temperatures and frequent rains accelerate water loss,softening,microbial attack and decay during storage.The aim of this study was to investigate and identify pathogens,reduce occurrence of diseases,and isolate and identify pathogenic bacteria as well as to detect the possible host.Initially,effects of light,temperature,pH and different C and N sources on colony growth was explored,and NaHCO3 and chitosan oligosaccharide(COS)were used for antibacterial test.The results showed that the pathogen was Byssochlamys sp.(B-TXL-02),which can cause disease in a variety of fruits,including pear(Pyrus spp.),apple(Malus domestica),peach(Prunus persica),cherry(Prunus avium),apricot(Prunus armeniaca),grape(Vitis vinifera),mango(Mangifera indica),banana(Musa spp.)and others fruits,indicating that the host had broad spectrum.Continuous illumination inhibited colony growth.Optimal factors for Byssochlamys sp.growth were temperature 30℃,pH 5,D-fructose and glucose for C source,beef extract and yeast extract for N source.When added into the potato dextrose agar,NaHCO3 and COS could significantly inhibit colony growth in the first 6d and the first 12 d,respectively,by inhibiting spore germination and inhibited mycelial growth.Treated with 0.3%NaHCO3+1.6% chitosan oligosaccharide,the postharvest rot rate of fruit was significantly reduced.This study provides a practical method for prevention and control of Byssochlamys sp.for Shengzhou Nane during postharvest storage.