文献类型：期刊文献

英文题名：Differential proteins from EVs identification based on tandem mass tags analysis and effect of Treg-derived EVs on T-lymphocytes in COPD patients

作者：Tao, Xuefang[1];Xu, Zhisong[1];Tian, Hai[2];He, Jingfeng[1];Wang, Guowen[1];Tao, Xuexia[3]

机构：[1]ShaoXing Univ, Affiliated Hosp, Dept Resp Med, 999 Zhongxing South Rd, Shaoxing 312000, Zhejiang, Peoples R China;[2]Shaoxing Univ, Med Coll, Dept Basic Med, 900 Chengnan Ave, Shaoxing 312000, Zhejiang, Peoples R China;[3]West Lake Univ, Hangzhou Peoples Hosp 1, Phase I Clin Res Ctr, 261 Huansha Rd, Hangzhou 310006, Zhejiang, Peoples R China

年份：2024

卷号：25

期号：1

外文期刊名：RESPIRATORY RESEARCH

收录：SCI-EXPANDED(收录号：WOS:001324831900007)、、Scopus(收录号：2-s2.0-85205254183)、WOS

基金：Not applicable.

语种：英文

外文关键词：Chronic obstructive pulmonary disease; T-lymphocyte subsets; Tregs; EVs; Tandem mass tags analysis; Proteomics analysis

外文摘要：BackgroundChronic obstructive pulmonary disease (COPD) is a widespread respiratory disease. This study examines extracellular vesicles (EVs) and proteins contained in EVs in COPD.MethodsBlood samples were collected from 40 COPD patients and 10 health controls. Cytokines including IFN-gamma, TNF-alpha, IL-1 beta, IL-6, IL-8, and IL-17, were measured by ELISA. Small EVs samples were extracted from plasma and identified by transmission electron microscope (TEM), nanoparticle tracking analysis (NTA), and Western blot. Protein components contained in EVs were analyzed by Tandem Mass Tags (TMT) to identify differential proteins. Treg-derived EV was extracted and added to isolated CD8+, Treg, and Th17 subsets to assess its effect on T-lymphocytes.ResultsELISA revealed higher levels of all cytokines and flow cytometry suggested a higher proportion of Treg and Th17 cells in COPD patients. After identification, TMT analysis identified 207 unique protein components, including five potential COPD biomarkers: BTRC, TRIM28, CD209, NCOA3, and SSR3. Flow cytometry revealed that Treg-derived EVs inhibited differentiation into CD8+, CD4+, and Th17 cells.ConclusionThe study shows that cytokines, T-lymphocyte subsets differences in COPD and Treg-derived EVs influence T-lymphocyte differentiation. Identified biomarkers may assist in understanding COPD pathogenesis, prognosis, and therapy. The study contributes to COPD biomarker research.