文献类型：期刊文献

英文题名：The ectopic expression of PttKN1 gene causes pleiotropic alternation of morphology in transgenic carnation (Dianthus caryophyllus L.)

作者：Meng, Lai-Sheng[1,2];Song, Jiang-Ping[3];Sun, Shao-Bo[4];Wang, Chong-Ying[1]

机构：[1]Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China;[2]Tongji Univ, Coll Life Sci, Shanghai 200092, Peoples R China;[3]Shaoing Univ, Sch YuanPei, Shaoxing 312000, Peoples R China;[4]Gansu Coll Tradit Chinese Med, Lanzhou 7300003, Peoples R China

年份：2009

卷号：31

期号：6

起止页码：1155

外文期刊名：ACTA PHYSIOLOGIAE PLANTARUM

收录：SCI-EXPANDED(收录号：WOS:000271067900008)、、Scopus(收录号：2-s2.0-70350582629)、WOS

基金：The authors thank Dr. Olof Olsson (Goteborg University, Goteborg, Sweden) for providing the plasmid containing 35S: PttKN1. This research was supported by the Natural Science Foundation of China (Grant No. 30470863).

语种：英文

外文关键词：PttKN1 gene; Dianthus caryophyllus L. (carnation); Novel lines; NPTII gene; The ectopic meristem formation

外文摘要：Carnation (Dianthus caryophyllus L.) is one of the most important ornamental plants in the world. Though morphological modification of carnation is very important to its commercial value, there have been no relevant reports until now. PttKN1 (Populus tremula x Tremuoides knotted1), isolated from the vascular cambial region of hybrid aspen, is a novel member of KNOX gene family. In this paper, we transformed 35S:PttKN1 to carnation via Agrobacterium tumefaciens. All primary transformants subsequently obtained were placed into phenotypic categories and self-pollinated. A total of 32 T0 progeny with aberrant phenotypes were obtained. PCR assay proved the validity of these transgenic plants. Phenotypes of 32 35S:PttKN1 plants were distinct from those of wild-type plants, including: (1) modification of phyllotaxis (15/32): wild-type carnation was with typical opposite phyllotaxis, while transgenic plants displayed tricussate whorled and multiple-cussate whorled phyllotaxis. Irregular modification of phyllotaxis was also observed; (2) modification of stem (9/32): wild-type stems were round; however, some transgenic plants exhibited much thicker and flatter stem; (3) the whole transgenic plants of carnation (8/32) became dwarf. These morphological modifications of carnation indicate that we have successfully attained some novel lines of carnation. These lines can have potential practical applications. In conclusion, the selection of stably genetic lines is discussed.