文献类型：期刊文献

英文题名：Apoptosis of human umbilical vein endothelial cells induced by artesunate

作者：Wu G.-D.[1,2];Zhou H.-J.[1];Wu X.-H.[1]

机构：[1]Zhejiang Univ, Coll Pharmaceut Sci, Dept Pharmacol & Toxicol, Hangzhou 310031, Peoples R China;[2]Shaoxing Univ, Dept Chem, Shaoxing 312000, Peoples R China

年份：2004

卷号：41

期号：6

起止页码：205

外文期刊名：VASCULAR PHARMACOLOGY

收录：SCI-EXPANDED(收录号：WOS:000226710800004)、、Scopus(收录号：2-s2.0-11844281505)、WOS

基金：We would like to acknowledge the contribution of Hong-yu Jia for the assistance with analysis of DNA fragmentation. This work was supported in part by a Grant-in-Aid for new drug research from national key lab of Chinese Academy of Sciences and by funds for Natural Science Foundation of Zhejiang Province (No. 303842).

语种：英文

外文关键词：artesunate; apoptosis; human umbilical vein endothelial cells; bcl-2; Bax; calcium

外文摘要：Artesunate (ART), a semi-synthetic derivative of artemisinin isolated from the traditional Chinese herb Artemisia annua. is an effective novel antimalarial drug. The present study investigated the apoptotic activity of artesunate in cultured human umbilical vein endothelial cell (HUVEC) by means of nuclear staining, DNA agarose gel electrophoresis, and flow cytometry. The observations also indicated that artesunate induced apoptosis of HUVEC in a concentration-dependent and time-dependent manner. A Western immunoblot analysis showed down-regulation of the bcl-2 protein and up-regulation of the bax protein in the artesunate-treated HUVEC. Ca2+ in cells was evaluated by fluorescent spectrophotometer using Fura 2-AM as probe. These results suggest that artesunate may be a potential apoptosis-inducing agent for endothelial cells. (C) 2004 Elsevier Inc. All rights reserved.