文献类型：期刊文献

英文题名：An integrated microfluidic cytosensor coupled with self-calibration electrochemical/colorimetric dual-signal output for sensitive and accurate detection of hepatoma cells

作者：Tang, Xiaoya[1];Tang, Junyan[1];Zhang, Qi[1];Suonanmu, Dongzhi[2];Zhang, Yanfei[2];Ren, Qingjia[2];Tao, Feifei[3];Li, Caolong[1,2];Wang, Fei[1]

机构：[1]China Pharmaceut Univ, Sch Sci, Dept Chem, Nanjing 211198, Peoples R China;[2]Univ Tibetan Med, Tibetan Med Res Inst, Tibet 850000, Peoples R China;[3]Shaoxing Univ, Dept Chem & Chem Engn, Shaoxing 312000, Peoples R China

年份：2024

卷号：403

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：SCI-EXPANDED(收录号：WOS:001149526000001)、、EI(收录号：20240115310368)、Scopus(收录号：2-s2.0-85181111800)、WOS

基金：We appreciate the financial support by the Science and Technology Plan Projects of Tibet Autonomous Region (XZ202301YD0018C) , Key Research and Development Program of Tibet Autonomous Region (XZ202301ZY0015G) and the Construction Project of Traditional Tibetan Medicine and Quality Control Laboratory Platform (KYPT2203) . The authors would also like to thank Shiyanjia Lab ( www.shiyanjia.com ) for the support of XPS test.

语种：英文

外文关键词：Microfluidic cytosensor; Electrochemistry/colorimetry; Dual-mode signal; Dual-site recognition; HepG2 cells

外文摘要：Hepatocellular carcinoma (HCC) is one of the highly prevalent and lethal malignancies around the world. As circulating tumor cells (CTCs) are regarded as critical indicators in the early diagnosis and prognosis of cancer, it is imperative to develop hepatoma cytosensor in liquid biopsy. Herein, this study introduces a microfluidic cytosensor for HepG2 cell detection, leveraging dual-site recognition and dual-mode signal readout to enhance specificity and accuracy. The cytosensor employs hyaluronic acid (HA) functionalized magnetic beads (MB-HA) and phenylboronic acid (PBA) modified iron metal organic framework (Fe-MOF-PBA) for magnetic isolation and signal labeling. Owing to the favorable peroxidase-mimic and electroactivity of Fe-MOF, the color of oxidized 3,3 ',5,5 '-tetramethylbenzidine (TMB) and the oxidation current of its amino-functionalized organic ligand were served as electrochemical/colorimetric (EC/CL) dual-signal for HepG2 quantification. This approach achieved a detection limit of 3 cells mL-1 (S/N = 3) and a broad linear range of 10-105 cells mL-1. Furthermore, the successful determination of HepG2 in clinical blood samples manifests the great prospect of the integrated microfluidic chip in precise, early point-of-care HCC diagnostics.