文献类型：期刊文献

英文题名：Assembly and co-occurrence patterns of rhizosphere bacterial communities are closely linked to soil fertility during continuous cropping of cut chrysanthemum (Chrysanthemum morifolium Ramat)

作者：Li, Jun[1];Meng, Feng[2];Jiang, Maibo[3];Zhang, Hanjie[1];Chu, Guixin[1];Tao, Rui[1,4]

机构：[1]Shaoxing Univ, Sch Life Sci, Shaoxing 312000, Zhejiang, Peoples R China;[2]Shaoxing Serv Ctr Environm Protect Sci & Technol, Shaoxing 312000, Zhejiang, Peoples R China;[3]Ningbo City Coll Vocat Technol, Ningbo 315100, Zhejiang, Peoples R China;[4]Shaoxing Univ, Sch Life Sci, Dept Environm Sci & Engn, Huancheng West Rd 508, Shaoxing 312000, Peoples R China

年份：2023

卷号：134

期号：8

外文期刊名：JOURNAL OF APPLIED MICROBIOLOGY

收录：SCI-EXPANDED(收录号：WOS:001049601700002)、、EI(收录号：20240715561307)、Scopus(收录号：2-s2.0-85168255481)、WOS

语种：英文

外文关键词：cut chrysanthemum rhizosphere; continuous cropping; soil fertility; bacterial community; co-occurrence network

外文摘要：Aims Continuous cropping is known to have profound effects on the soil microbial community in different planting systems. However, we lack an understanding of how different years of continuous cropping affects rhizosphere soil bacterial community co-occurrence pattern and assembly processes in the cut chrysanthemum (Chrysanthemum morifolium Ramat.) field. Methods and results We collected the soils from cut chrysanthemum rhizospheres with planting for 1 year (PY1) and continuous cropping for 6 years (CY6) and 12 years (CY12). Real-time quantitative PCR and flow cytometry (FCM) techniques were used to test the 16S rRNA gene copy number and bacterial cell count, respectively. The bacterial community structure was analysed by using high-throughput sequencing technology. The CY12 had a significantly decreased soil fertility index and rhizosphere bacterial living cell counts and gene copy numbers compared to CY6 and PY1 (P < 0.05). The rhizosphere bacterial community dissimilarity increased as the continuous cropping years increased. Three main ecological clusters (modules #1, #2, and #3) were observed in the bacterial co-occurrence network across all samples, and only the relative abundance of module #1 (enriched in the CY12) was significantly correlated with soil fertility (P < 0.05). Moreover, the rhizosphere bacterial community assembly was primarily governed by the deterministic process under 12 years of continuous cropping. Conclusions Soil fertility decline correlates with ecological network modularization and the deterministic assembly process of the rhizosphere bacterial community of cut chrysanthemum during continuous cropping.