文献类型：期刊文献

中文题名：孑遗植物桫椤内生真菌的多样性与群落组成

英文题名：Species diversity and community composition of endophytic fungi from Alsophila spinulosa

作者：臧威[1];Luke Saye Nenwon KRUA[1];沈赤[1];杜贞娜[1];孙翔[2];孙剑秋[1];傅建伟[1]

机构：[1]绍兴文理学院生命科学学院,浙江绍兴312000;[2]中国科学院微生物研究所真菌学国家重点实验室,北京100101

年份：2020

卷号：39

期号：4

起止页码：731

中文期刊名：菌物学报

外文期刊名：Mycosystema

收录：CSTPCD、、北大核心2017、CSCD2019_2020、北大核心、CSCD

基金：Supported by the National Natural Science Foundation of China(31500020).

语种：中文

中文关键词：孑遗植物;真菌资源;分类单元;铺散内曼菌

外文关键词：relic plant;fungal resources;taxa;Nemania diffusa

中文摘要：桫椤为木本蕨类,在世界范围内属于濒危的孑遗植物,关于桫椤内生真菌的研究还未见报道。本文基于形态学特征和DNA序列分析技术,探讨了桫椤内生真菌多样性和群落组成。研究发现,桫椤组织中蕴藏着丰富的真菌资源,内生真菌定殖率达到87.6%;从桫椤的234个生活组织块中,分离到内生真菌291株,被鉴定为64个分类单元;桫椤组织中常见真菌种类大约占内生真菌的60%,其中铺散内曼菌Nemania diffusa表现出明显的分布优势;内生真菌总体多样性指数达到3.53的较高水平,并且表现出叶轴>根部>羽片>叶柄>茎皮的分布规律;分析不同组织中内生真菌的相似性系数和聚类结果,发现桫椤的组织性质影响内生真菌的组成和分布;根据物种累积曲线,现有的研究样本中超过80%的内生真菌已经被分离到,研究结果能够反映桫椤内生真菌的多样性和群落组成。通过分析桫椤的叶柄凋落物中真菌定殖率、丰度、多样性、相似性和真菌种类等特征性数值,表明凋落的桫椤组织中真菌的分布特征已经发生了明显改变。

外文摘要：Tree fern Alsophila spinulosa is an endangered relic plant in the world. The research on endophytic fungi in A. spinulosa has not been reported yet. Here, the diversity and community composition of endophytic fungi in A. spinulosa were investigated based on morphological characteristics and phylogenetic analyses of DNA sequence data. Our results revealed that A. spinulosa concealed abundant fungal species and the colonization rate of endophytic fungi reached 87.6%. A total of 291 isolates of endophytic fungi belonging to 64 different taxa was obtained from 234 living tissue segments of A. spinulosa;of which approximately 60% were common species, and Nemania diffusa exhibited predominant. Diversity index of endophytic fungi was as high as 3.53, and varied with different microhabitats ranged from high to low in order of rachis > root > pinna > petiole > bark. The species composition and distribution of endophytic fungi in the plants might be affected by tissue properties of A. spinulosa, as indicated by the analysis of cluster and Sorenson’s similarity coefficient. More than 80% of endophytic fungi in A. spinulosa sample had been isolated according to the analyses of species accumulation curves, and our result could reflect the diversity and community composition of the fungi. Analysis results of colonization rate, species richness, diversity, similarity of community in the petiole litter of A. spinulosa indicated that the community of fungi in the litter had changed considerably.