文献类型：期刊文献

中文题名：丹血通注射液对大鼠睾丸扭转复位后的保护作用

英文题名：Protective effect of Danxuetong injection against testicular injury after testis torsion/detorsion in rats

作者：杨斐[1];杨超玲[1];余雯文[1];俞子锋[1];刘荣华[1];倪培昀[1];张金萍[1]

机构：[1]绍兴文理学院医学院临床医学系

年份：2015

卷号：21

期号：9

起止页码：828

中文期刊名：中华男科学杂志

外文期刊名：National Journal of Andrology

收录：MEDLINE(收录号：26552218)、CSTPCD、、北大核心2014、CSCD2015_2016、Scopus、北大核心、CSCD、PubMed

基金：绍兴文理学院医学院学生科研项目(201424)~~

语种：中文

中文关键词：丹血通注射液;睾丸扭转;缺血再灌注;氧自由基

外文关键词：Danxuetong injection; testicular torsion; ischemia-reperfusion injury; oxygen free radical

中文摘要：目的:研究丹血通注射液对大鼠睾丸扭转复位后缺血再灌注损伤的保护作用。方法:将32只4周龄健康SD雄性大鼠,随机分为丹血通注射液单次给药组、丹血通注射液连续给药组、生理盐水组和假手术组,每组8只。建立单侧睾丸扭转复位动物模型(720°,2 h)。于术后6周处死大鼠,计算睾丸系数,测定精子计数和精子活动率,检测睾丸组织总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)活性与丙二醛(MDA)含量。结果:丹血通注射液单次给药组、连续给药组手术侧睾丸系数(0.35±0.04、0.40±0.06)比同侧生理盐水组(0.11±0.03)均显著升高(P均<0.05);精子计数[(1.44±0.50)、(3.00±1.28)×109/ml]和精子活动率[(39.63±5.04)%、(76.31±3.67)%]比同侧生理盐水组[(0.46±0.10)×109/ml、(13.63±14.04)%]均显著升高(P均<0.05);两给药组SOD(116.25±8.83、133.20±13.84)、T-AOC(13.34±5.81、19.21±5.69)活性和MDA含量(20.94±5.65、15.02±1.03)比同侧生理盐水组SOD(72.76±5.58)、T-AOC(5.58±1.07)活性升高和MDA含量(42.38±8.94)显著降低(P均<0.05)。且连续给药组手术侧与单次给药组睾丸系数、精子计数、精子活动率、SOD、MDA、T-AOC比较差异均有显著性(P均<0.05)。结论:丹血通注射液可有效清除氧自由基,通过抗氧化作用可以提高精子的活动率和数量,对青春前期大鼠睾丸缺血再灌注损伤具有明显的保护作用,对其对侧睾丸亦有一定的保护作用,且连续给药效果明显优于单次给药。

外文摘要：Objective： To investigate the protective effect of Danxuetong injection （ DXT, a combination of Danshen and Xueshuantong injections） against testicular ischemia-reperfusion injury following testis torsion/detorsion in rats. Methods： Thirty-two 4- week-old healthy male SD rats were randomly divided into four groups of equal number ： sham operation, normal saline, single DXT injection, and successive DXT injection. The rat models of testicular ischemia-reperfusion injury were established by 2-hour 720-degree torsion/detorsion of the unilateral testis. At 6 weeks after modeling, the rats were killed and their testes were harvested for measurement of testicular coefficients, sperm counts, sperm motility, and the levels of total anti-oxidative capacity （ T-AOC）, superoxide dismutase （SOD）, nitric oxide synthase （NOS）, and malondialdehyde （MDA） in the testis tissue. Results： Compared with the rats of the normal saline group, those of the single DXT injection and successive DXT injection groups showed significant increases in the testicular coefficient （0.11 ± 0.03 vs 0. 35 ±0. 04 and 0.40 ± 0.06, P 〈 0.05 ）, sperm count （ [ 0.46 ± 0.10 ] vs [ 1.44 ± 0.50 ] and [3.00±1.28] ×10^9/ml, P〈0.05）, sperm motility （[13.63±14.04] vs [39.63 ±5.04] and [76.31±3.671%, P〈0.05）, the activity of SOD （72.76 ± 5.58 vs 116.25 ±8.83 and 133.20 ±13.84, P 〈 0.05 ）, and the level of T-AOC （5.58 ± 1.07 vs 13.34 ± 5.81 and 19.21± 5.69, P 〈 0.05）, but a remarkable decrease in the content of MDA （42.38 ±8.94 vs 20.94 ± 5.65 and 15.02 ± 1.03, P 〈 0.05 ） in the injured testes. Conclusion ： DXT can effectively rid the testis tissue of oxygen free radicals, improve sperm count and motility by antioxidation, and protect the testis tissue of prepubertal rats against testicular ischemia-reperfusion injury after testis torsion/detorsion. It also has a protective effect on the contralateral testis, and successive injection has a better effect than single injection of DXT.