文献类型：期刊文献

英文题名：Carp edema virus, an emerging threat to the carp (Cyprinus carpio) industry in China

作者：Zhang, Xiaodong[1];Ni, Yingying[1];Ye, Jian[2];Xu, Hui[3];Hou, Yuefeng[1];Luo, Wen[1];Shen, Wenying[1]

机构：[1]Shaoxing Univ, Coll Life Sci, 900 Chengnan Ave, Shaoxing 312000, Zhejiang, Peoples R China;[2]Hangzhou Aquacultural Tech Extending Ctr, Hangzhou 310001, Zhejiang, Peoples R China;[3]Shaoxing Entry Exit Inspect & Quarantine Bur, Shaoxing 312000, Zhejiang, Peoples R China

年份：2017

卷号：474

起止页码：34

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:000402346200005)、、WOS

基金：This work was supported by the National Natural Science Foundation of China (31201930), National Public Service Sectors (Agriculture) Research Project of China (201203081) and Public Projects of Shaoxing, China (2015B70024).

语种：英文

外文关键词：Koi sleepy disease; Carp edema virus; Cyprinus carpio; Outbreak; China

外文摘要：Koi sleepy disease (KSD), also known as carp edema virus (CEV) disease, is an important, largely acute infection of common carp and ornamental koi (Cyprinus carpio). Since the first report of KSD in Japan in the 1970s, the disease has spread to many countries including England, France, the Netherlands, Germany, Italy, the Czech Republic, Austria, Poland, and India. In the spring of 2015, a koi farm in Hangzhou experienced an outbreak of an infectious disease. In this study we conducted clinical, histopathological, and molecular diagnostic analyses to determine the cause of the outbreak. CEV was found to be the causative agent of the disease. Moreover, PCR sequencing and phylogenetic analyses revealed that CEV was the extremely likely etiological agent of the mortality in this outbreak, which was further confirmed by sequence analysis of the PCR products obtained after nested PCR of the degenerate PCR. We also found that temperature is an important factor affecting carp morbidity and mortality in CEV infection. This is the first report of the detection of CEV in relation to KSD outbreaks in China. Our findings highlight the need for research on the CEV genome to better characterize the spread of CEV.