文献类型：期刊文献

英文题名：Dual-Targeting Nanoprobe for Early Diagnosis of Pheochromocytoma Though Coinstantaneous Identification of Circulating Tumor Cells

作者：Liu, Xiaoya[1];Zhang, Xicheng[1];Tang, Junyan[1];Meng, Yang[1];Zhao, Liping[1];Shi, Wenjing[1];Tao, Feifei[2];Kan, Zigui[1];Wang, Fei[1];Li, Caolong[1]

机构：[1]China Pharmaceut Univ, Sch Sci, Key Lab Biomed Funct Mat, Nanjing 211198, Peoples R China;[2]Shaoxing Univ, Dept Chem & Chem Engn, Shaoxing 312000, Peoples R China

年份：2021

卷号：93

期号：26

起止页码：9036

外文期刊名：ANALYTICAL CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000672115800005)、、EI(收录号：20212910648928)、Scopus(收录号：2-s2.0-85110084858)、WOS

基金：This work was supported by the National Key Research and Development Program (2019YFC0312602), the Natural Science Foundation of China (51372154), and National Innovation and Enterpreneurship Training Program for Undergraduate (202110316165).

语种：英文

外文关键词：Cells - Cytology - Iron oxides - Magnetite - Nanoprobes - Silica - Silver compounds - Tumors

外文摘要：Circulating tumor cells (CTCs) play a pivotal role in the early diagnosis of pheochromocytoma (PCC). Herein, we fabricated a new dual-targeting nanoprobe for coinstantaneous identification of rare PCC-CTCs from peripheral blood via targeting the norepinephrine transporter (NET) and somatostatin receptor SSTR2 overexpressed on the surface of PCC cells. Meta-iodobenzylguanidine (MIBG) functionalized magnetic Fe3O4 and octreotide (DOTA) decorated signal amplification Ag@SiO2 nanosphere were used to capture and detect PCC-CTCs by binding to NET and SSTR2. The proposed dual-targeting sensor achieved good reproducibility and high sensitivity for the monitoring of PC12 in the concentration range from 5 to 5 X 10(4) cells mL(-1), with detection limits of 2 cell/mL. This strategy opens a new approach for simple, sensitive, and rapid determination of PCC biomarkers, which shows great potential in early diagnosis, prognosis, and therapeutic evaluation of PCC.