文献类型：期刊文献

中文题名：纳米二氧化铈对神经细胞PC12及SH-SY5Y活力的影响

英文题名：Effects of CeO 2 nanoparticles on the viabilities of neural PC12 and SH-SY5Y cells

作者：许逸岚[1];赵镘娜[1];朱诗涵[1];韩瑜颖[1];章佳瑜[1];沈铄恒[1];俞樟森[1];张衡[1]

机构：[1]绍兴文理学院医学院基础医学部,浙江绍兴312000

年份：2020

卷号：36

期号：1

起止页码：62

中文期刊名：中国应用生理学杂志

外文期刊名：Chinese Journal of Applied Physiology

收录：CSTPCD、、北大核心2017、Scopus、CSCD2019_2020、北大核心、CSCD、PubMed

基金：国家自然科学基金项目(31800891);浙江省新苗人才计划项目(2018R432029,2019R432025)。

语种：中文

中文关键词：纳米CeO;2;活性氧;神经细胞;细胞活力;阿尔茨海默病

外文关键词：CeO 2 nanoparticles;reactive oxygen species;neural cells;cell viability;Alzheimer’s disease

中文摘要：目的:探究纳米二氧化铈(CeO 2)对神经细胞PC12与SH-SY5Y活力的影响。方法:合成纳米CeO 2材料,并对其结构进行表征,性能进行评估。用不同终浓度(1、2.5、5、10、25、50、75、100、150μg/ml)的纳米CeO 2分别处理PC12细胞与SH-SY5Y细胞24 h,使用MTT法检测其细胞活力。然后使用活性氧清除剂NAC与纳米CeO 2共孵育处理PC12细胞与SH-SY5Y细胞,并用DCFH-DA探针染色,在荧光倒置显微镜下观察各组细胞的数量及其荧光强度。对实验数据采用单因素方差(one-way ANOVA)分析。结果:纳米CeO 2处理后,PC12细胞(P<0.01)与SH-SY5Y细胞(P<0.01)的活力都明显下降,与对照组差异明显。DCFH-DA探针染色后,发现纳米CeO 2的浓度越高,荧光强度越强,表明有活性氧(ROS)的产生。活性氧清除剂NAC与纳米CeO 2(100μg/ml)共同处理PC12后,荧光强度明显减弱。与25μg/ml(P<0.01)、50μg/ml(P<0.01)、75μg/ml(P<0.01)、100μg/ml(P<0.01)纳米CeO 2处理组相比,共同处理组细胞活力明显增加。结论:纳米CeO 2对神经细胞PC12与SH-SY5Y的活力有明显的抑制作用,其机制可能与ROS有关。

外文摘要：Objective:To investigate the effects of cerium oxide(CeO 2)nanoparticles on the viabilities of nerve cells PC12 and SH-SY5Y.Methods:CeO 2 nanoparticles were synthesized,structures were characterized and properties were evaluated.PC12 cells and SH-SY5Y cells were treated with CeO 2 nanoparticles at different concentrations(1,2.5,5,10,25,50,75,100,150μg/ml)for 24 h and the cell viability was measured by MTT assay.Then PC12 cells and SH-SY5Y cells were co-treated with CeO 2 and active oxygen scavenger NAC and the cells were stained with DCFH-DA probe for ROS.The number of cells and the fluorescence intensity were observed under a fluorescent inverted microscope.Differences were assessed by one-way ANOVA.Results:After treatment with CeO 2 nanoparticles,the viabilities of both PC12 cells(P<0.01)and SH-SY5Y cells(P<0.01)were decreased comparing with the control group.After staining with DCFH-DA probe,the fluorescence intensity of the nerve cells was enhanced depending on the concentration of CeO 2 nanoparticles suggesting that CeO 2 induced the generation of reactive oxygen species(ROS).The fluorescence intensity of PC12 cells was decreased after CeO 2 nanoparticles(100μg/ml)co-treatment with active oxygen scavenger NAC.Compared with CeO 2 nanoparticles alone at 25μg/ml(P<0.01),50μg/ml(P<0.01),75μg/ml(P<0.01),100μg/ml(P<0.01),the cell viability was significantly increased after co-treatment with NAC.Conclusion:CeO 2 nanoparticles has a negative effect on the viabilities of nerve cells PC12 and SH-SY5Y,and the effect might be depend on ROS.