文献类型：期刊文献

英文题名：Selection and application of aptamers with high-affinity and high-specificity against dinophysistoxin-1

作者：Li, Zhen[1];Hu, Bo[1,2];Zhou, Rong[1];Zhang, Xiaojuan[1,4];Wang, Ruizhe[3];Gao, Yun[1];Sun, Mingjuan[1];Jiao, Binghua[1,2];Wang, Lianghua[1]

机构：[1]Navy Med Univ, Coll Basic Med Sci, Dept Biochem & Mol Biol, Shanghai 200433, Peoples R China;[2]Naval Special Med Ctr, Dept Marine Biomed & Polar Med, Shanghai 200433, Peoples R China;[3]Second Mil Med Univ, Changzheng Hosp, Dept Orthoped, Spine Ctr, 415th Feng Yang Rd, Shanghai 200003, Peoples R China;[4]Shaoxing Univ, Dcoll Med, 900th Chengnan Ave, Shaoxing 312000, Zhejiang, Peoples R China

年份：2020

卷号：10

期号：14

起止页码：8181

外文期刊名：RSC ADVANCES

收录：SCI-EXPANDED(收录号：WOS:000521329900021)、、EI(收录号：20201108291552)、Scopus(收录号：2-s2.0-85081129865)、WOS

基金：This work was supported by the China National Major Scientific and Technological Special Project for Significant New Drugs Development (No. 2018ZX09J18112) and the National Natural Science Foundation of China (No. 41706156).

语种：英文

外文关键词：Binding energy - Electric current measurement

外文摘要：Diarrhetic shellfish toxins (DSTs) are marine toxins distributed widely in the world, which pose a major threat to the health of mankind. Dinophysistoxin-1 (DTX-1) has the most potent toxicity in DSTs. However, the current detection methods have ethical problems and technical defects. Further research is needed, to develop a more suitable alternative to the supervision system. In this work, we successfully obtained an aptamer with high affinity and specificity bound to DTX-1 for the first time. After optimization, a core sequence of the aptamer with a higher K-D of 64 nM was obtained, while the binding mode of the core sequence and DTX-1 was explored. Based on this aptamer, we developed a biolayer interferometry (BLI) biosensor platform for DTX-1 detection. The aptasensor exhibited a broad detection range from 40 to 600 nM DTX-1 (linear range from 80 to 200 nM), and the low detection limit was 614 pM. Morever, the aptasensor showed good reproducibility and stability, which indicated that this novel aptasensor had broad development prospects for the sensitive and rapid detection of DTX-1.