文献类型：期刊文献

中文题名：同型半胱氨酸介导的内质网应激对肝细胞脂质代谢的影响

英文题名：Effect of homocysteine-mediated endoplasmic reticulum stress on lipid metabolism in hepatocytes

作者：晏春根[1];任光圆[1];朱冬芳[1];倪培华[2];周惠娟[3]

机构：[1]绍兴文理学院医学院;[2]上海交通大学医学院检验系;[3]上海交通大学医学院附属瑞金医院感染科

年份：2007

卷号：23

期号：12

起止页码：2414

中文期刊名：中国病理生理杂志

外文期刊名：Chinese Journal of Pathophysiology

收录：CSTPCD、、北大核心2004、CSCD2011_2012、北大核心、CSCD

基金：浙江省高校青年教师资助项目(No浙教高科[2004]281号);浙江省教育规划课题资助项目(No20041234)

语种：中文

中文关键词：高半胱氨酸;内质网;应激;脂质代谢

外文关键词：Homocysteine ; Endoplasmic reticulum ; Stress ; Lipid metabolism

中文摘要：目的:探讨同型半胱氨酸(Hcy)介导的内质网应激对肝细胞脂质代谢的影响。方法:5mmol/LHcy与HepG2细胞共孵育,检测细胞内Hcy、甘油三酯、胆固醇的含量。高蛋氨酸饮食诱导小鼠高Hcy血症,分析肝细胞内甘油三酯(TGE)、胆固醇(CHO)含量的变化,检测内质网应激蛋白葡萄糖调节蛋白78(GRP-78)、胆固醇调节元件结合蛋白(SREBP-1)mRNA及蛋白表达的变化。结果:与Hcy孵育后,各时点HepG2细胞内Hcy、脂质(TGE、CHO)含量均显著升高(P<0.05,vs0h)。高蛋氨酸饮食小鼠各时点血浆Hcy、肝细胞内TGE、CHO含量均显著高于0周(P<0.05),而血浆TGE、CHO含量升高并不明显(P>0.05,vs0周);肝组织内质网应激蛋白GRP-78、SREBP-1mRNA及其蛋白表达均显著高于0周(P<0.05)。结论:Hcy诱导的内质网应激可致内源性胆固醇调节通路失调,并使肝细胞合成、摄取甘油三酯、胆固醇增加。

外文摘要：AIM： To explore the effect of homocysteine （Hey） - mediated endoplasmic reticulum stress on lipid metabolism in hepatocytes. METHODS： HepG2 cells used in the study were treated with 5 mmol/L Hcy. The concentrations of Hcy, triglycerides （TGE） and cholesterol （CHO） in the cells were measured. In high methionine diet - induced hyperhomocysteinemia C57BL/6 mice, the concentrations of TGE and CHO in hepatocytes were analyzed. The mRNAs and proteins expressions of glucose - regulated protein 78 （ GRP - 78） and sterol regulatory element binding protein （ SREBP - 1 ） were also assessed. RESULTS ： The concentrations of Hcy and lipids （TGE, CHO） in HepG2 cells at different time point were elevated after treated with 5 mmol/L Hcy （ P 〈0.05, vs 0 hour）. The levels of plasma Hcy and TGE or CHO in hepatocytes of high methionine diet mice at different time point were significantly increased （ P 〈0.05 ,vs 0 week）. However, the TGE and CHO levels in plasma had no change （ P 〉 0.05, vs 0 week）. The mRNAs and proteins expressions of GRP - 78 and SREBP - 1 in mice at different time point after high methionine diet were higher than that at 0 week （ P 〈 0. 05）. CONCLUSION ： It suggests that Hcy - induced endoplasmic reticulum stress causes dysregulation of the endogenous sterol response pathway, also increases hepatic biosynthesis and uptake of TGE and CHO.