文献类型：期刊文献

中文题名：阳离子树脂吸附分离-HPLC测定乳酸司帕沙星脂质体的包封率

英文题名：Determination of Entrapment Efficiency in Sparfloxacin Lactate Liposomes by Cation Exchange Resin-HPLC

作者：刘利萍[1];李益民[1];董瑛瑛[2]

机构：[1]绍兴文理学院化学化工学院药学系,浙江绍兴312000;[2]绍兴人民医院药学部,浙江绍兴312000

年份：2008

卷号：43

期号：14

起止页码：1082

中文期刊名：中国药学杂志

外文期刊名：Chinese Pharmaceutical Journal

收录：CSTPCD、、北大核心2004、Scopus、CSCD2011_2012、北大核心、CSCD、PubMed

语种：中文

中文关键词：乳酸司帕沙星;脂质体;包封率;阳离子树脂柱;高效液相色谱法

外文关键词：sparfloxcain lactate ; liposomes ; entrapment efficiency ; cation exchange resin column ; HPLC

中文摘要：目的建立乳酸司帕沙星脂质体包封率的测定方法、方法采用HPLC测定药物含量,用Hypersil ODS2柱(4.6 mm×250mm,5μm),0.2mol·L^(-1)醋酸铵溶液(用冰醋酸调pH至3.5)-乙晴(70:30)为流动相,流速为1.0mL·min^(-1),检测波长为298nm;采用5mL阳离子树脂柱分离脂质体中的游离药物,加样量0.2mL,用13mL去离子水洗脱,测定包封率。结果乳酸司帕沙星在4.2～50.4mg·L^(-1)内与峰面积线性关系良好(r=1.000),精密度RSD小于1.3%,回收率为99.96%～102.1%(RSD为0.9%～1.3%,n=3);5mL阳离子树脂柱对游离药物的平均吸附率大于96.4%,且对脂质体的洗脱率大于97.4%,可以将脂质体与游离药物分离。结论采用阳离子树脂吸附分离-HPLC测定乳酸司帕沙星脂质体中药物包封率简便快速,结果较可靠。

外文摘要：OBJECTIVE To establish cation exchange resin-HPLC method for the determination of entrapment efficiency in sparfloxacin lactate liposomes. METHODS The content of sparfloxaein lactate was determined by HPLC with Hypersil ODS2 column （4. 6 mm ×250 mm,5 μm）. The mobile phase consisted of 0. 2 mol·L-1 ammonium acetate solution （ adjusted to pH 3.5 with acetic acid ）- acetonitrile （70： 30） , at a flow- rate of 1.0 mL · min-1. The UV detection was set at 298 nm. The 5 ml, column with cation exchange resin was adopted to separate free sparfloxcain lactate from liposomes dispersions. The 0. 2 mL sample was added and washed through resin column with 13 mL deionized water. RESULTS The linear-calibration curves were obtained in the concentration range of 4. 2-50. 4 mg· L-1（ r = 1. 000）. The precision （RSD） was below 1.3%. The recoveries of sparfloxacin lactate with blank liposomes were 99. 96% - 102. 1% （ n = 3 ）. The free sparfloxacin lactate was well separated from liposnmes dispersions and was adsorbed up to 96.4% by cation exchange resin, and the average recover）, of liposomes were more than 97.4%. CONCLUSION This method is technically simple, rapid and efficient and is proved to be suitable for separating and determining entrapment efficiency of cationic drug liposomes.