文献类型：期刊文献

英文题名：Isolation, identification and pathogenesis study of Vibrio diabolicus

作者：Song, Jiayao[1];Liu, Xiaoyu[1];Wu, Cuiling[1];Zhang, Yu[1];Fan, Ke[1];Zhang, Xiaodong[2];Wei, Yongwei[1]

机构：[1]Shaoxing Univ, Sch Med, 900 Chengnan Ave, Shaoxing 312000, Zhejiang, Peoples R China;[2]Shaoxing Univ, Sch Life Sci, Shaoxing 312000, Zhejiang, Peoples R China

年份：2021

卷号：533

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:000605009800003)、、WOS

基金：This study was supported by the National Natural Science Foundation of China (31402218, 31201930), the Natural Science Foundation of Zhejiang Province (LY14C180001) and the National Innovation and Entrepreneurship Training Program for Undergraduates (201910349032). The authors declare that they have no conflicts of interest.

语种：英文

外文关键词：V. diabolicus; Gomphina aequilatera; Pathogenicity; Virulence

外文摘要：A strain of Vibrio diabolicus named SX-2018 was isolated from Callista chinensis in the Zhejiang coastal region of China. The isolate was identified as V. diabolicus by sequencing of the 16S rRNA, housekeeping genes and subclade-associated genes. Antibiotic resistance as well as biochemical and growth characteristics were determined. Animal studies were performed to evaluate the in vivo virulence of SX-2018. The results indicated that SX-2018 was highly related to the known deep-sea hydrothermal vent V. diabolicus and V. diabolicus from Homo sapiens. Artificial infection showed that SX-2018 exhibited strong pathogenicity toward Gomphina aequilatera, but it did not express pathogenicity toward Mactra veneriformis Reeve, Callista chinensis, Mactra chinensis Philippi, Ruditapes philippinarum, Cyclina sinensis, Dosinia corrugate Meretrix meretrix, Tegillarca granosa, Sinonovacula constricta or Danio rerio. Mortality in Gomphina aequilatera at 25 degrees C and 15 degrees C was 100% and 27%, respectively. Virulence-related gene sequence analysis showed that SX-2018 encoded the tlh gene but lacked tdh and trh genes. TIh and hemolysin coregulated protein genes of SX-2018 shared 99.36-99.79% homology to the deep-sea V. diabolicus strain, 98.65.36-99.79% homology to the V. diabolicus strain from Homo sapiens, and 97-97.79% and 84-89.79% similarity to Vibrio alginolyticus and Vibrio parahaemolyticus, respectively. This study will aid in understanding the evolutionary relationship between V. diabolicus and related pathogenic Vibrio species.