文献类型：期刊文献

英文题名：The Endosperm-Specific Expression of YUCCA Genes Enhances Rice Grain Filling

作者：Jiang, Huijun[1];Zhai, Kaien[3];Ye, Xiaofan[3];Hu, Tianwei[2];Jiang, Jieming[4];Dong, Xiaoqiu[1];Pan, Weihuai[1];Pan, Jianwei[2];Shou, Jianxin[1]

机构：[1]Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Peoples R China;[2]Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Zhejiang, Peoples R China;[3]Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China;[4]South China Normal Univ, Sch Life Sci, Guangzhou 510631, Peoples R China

年份：0

外文期刊名：PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY

收录：SCI-EXPANDED(收录号：WOS:000810725100001)、、WOS

基金：This work was supported by the National Natural Science Foundation of China (Grant Nos. 31801193, 31820103008, 91754104, and 31670283) and the Fundamental Research Funds for the Central Universities (No. lzujbky-2020-it13).

语种：英文

外文关键词：Auxin content; grain fi lling; IAA biosynthesis; rice; seed-setting rate; YUCCA genes

外文摘要：Grain filling is a crucial process that affects yield in rice (Oryza sativa L.). Auxin biosynthesis and signaling are closely related to rice yield; therefore, it is important to understand the effects of auxin biosynthesis on rice grain filling to improve crop yield. In this study, we used physiological and molecular strategies to identify the roles of auxin in rice grain filling. Exogenous application of auxin (IAA) or auxin analogues (2, 4-D) to young spikelets and flag leaves improved the seed-setting rate and yield per spike. Furthermore, real-time quantitative PCR assays confirmed that nine members of the OsYUCCA family of auxin biosynthetic genes were upregulated during grain filling, implication that auxin biosynthesis plays a major role in grain development. The specific expression of either Arabidopsis AtYUCCA1 or OsYUCCA2 in the endosperm or leaves resulted in increased expression of OsIAA genes and auxin content of seeds, as well as increased grain filling and seed-setting rate. This result establishes that the auxin content in grains and leaves is important for grain development. Our findings further highlight the potential applications for improving rice yield by elevating targeted gene expression in specific tissues.