文献类型：期刊文献

英文题名：A ratiometric fluorescent probe based on quinoline for monitoring and imaging of Leucine aminopeptidase in liver tumor cells

作者：Du, Kui[1];Sheng, Li[2];Luo, Xiang[1];Fan, Gang[2];Shen, Dadong[2];Wu, Chunlei[1];Shen, Runpu[1]

机构：[1]Shaoxing Univ, Sch Chem & Chem Engn, Shaoxing 312000, Peoples R China;[2]Zhejiang Med Co Ltd, Res & Dev Ctr, Shaoxing 312500, Peoples R China

年份：2021

卷号：249

外文期刊名：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

收录：SCI-EXPANDED(收录号：WOS:000609024100015)、、EI(收录号：20205209696148)、Scopus(收录号：2-s2.0-85098203654)、WOS

基金：This work was supported by Start up fund for doctoral research of Shaoxing University (20185020), Zhejiang public welfare fund project (No. LGG19B02002).

语种：英文

外文关键词：Leucine aminopeptidase; Liver cancer; Quinoline; Ratiometric; Hepatoma-specific

外文摘要：Leucine aminopeptidase (LAP) is known as an important potential biomarker for liver malignancy and it is urgent to develop an intuitive and effective method to monitor the activity of LAP in liver cancer. Although, numerous LAP fluorescent probes had been developed, it is still a challenge to detect LAP activity in liver cancer. Herein, combained with the DFT, we reported a novel galactose-appended hepatoma-specific ratiometric fluorescent probe (Gal-QL-Leu) based on quinoline group for imaging and tracing LAP in liver tumor cells. Probe Gal-QL-Leu demonstrated a obvious ratiometric characteristics, better selectivity, good biocompatibility and high sensitivity. Moreover, the selective imaging of LAP in HepG2, HCT116, A549 and HeLa cells had been achieved with probe Gal-QL-Leu, demonstrating good application prospect in the detection of LAP activity in liver tumor cells. (C) 2020 Published by Elsevier B.V.