文献类型：期刊文献

中文题名：响应面法优化纤维素酶提取香榧假种皮多酚类物质

英文题名：Optimization of Cellulase Extraction of Polyphenols from the Episperm of Torreya grandis by Response Surface Methodology

作者：冯孝杰[1];沈倩婷[1];陈犇[1];陈俊沲[1];周瑾[1];孙小红[1]

机构：[1]绍兴文理学院元培学院,绍兴312000

年份：2020

卷号：0

期号：2

起止页码：54

中文期刊名：食品工业

外文期刊名：The Food Industry

收录：北大核心2017、北大核心

基金：浙江省自然科学基金项目(2019C32032);绍兴文理学院元培学院药学特色专业(20160002).

语种：中文

中文关键词：香榧假种皮;纤维素酶;超声波辅助;多酚;提取;响应面法

外文关键词：episperm of Torreya grandis;cellulase;ultrasound-assisted;polyphenols;extraction;response surface methodology

中文摘要：以香榧假种皮为原料,利用纤维素酶法提取香榧假种皮中多酚类物质,在单因素试验基础上,通过DesignExpert统计分析软件中Box-Behnken试验设计方法,设计三因素三水平响应面试验,建立超声波辅助纤维素酶法提取香榧假种皮中多酚的二次多项数学模型,以多酚得率为响应值作响应面和等高线,最优浸提条件为:提取溶剂采用50%乙醇、纤维素酶0.4 mL (42.7 U/mL),提取温度59.45℃、pH 6.45、料液比1︰17.31 g/mL。在此条件下提取物多酚得率为22.45 mg/g。

外文摘要：Response surface methodology was employed to optimize the cellulase-assisted extraction of polyphenols from the episperm of Torreya grandis. On the basis of single factor experiment, a regression model was established based on a three-variable, three-level quadratic universal rotation combination design by Box-Behnken test design method of designexpert statistical analysis software, and ultrasonic-assisted cellulase method was established to extract polyphenols from the episperm of Torreya grandis. Response surface and contour plots were drawn for the extraction efficiency of polyphenols as a function of three operating parameters. The optimum extraction condition was found to be 50% ethanol as extraction solvent, 0.4 mL cellulase(42.7 U/mL), extraction temperature 59.45 ℃, pH 6.45, and solid-liquid ratio 1︰17.31 g/mL. Under this condition, the yield of polyphenols was 22.45 mg/g.