文献类型：期刊文献

英文题名：Characterization of the saponin hydrolases involved in the biotransformation of saponins with Trichoderma reesei

作者：Zhu, Yuling[1]; Zheng, Tianxiang[1]; Yu, Lidan[1]; Zhao, Bin[2]

机构：[1] College of Life Sciences, Shaoxing University, Shaoxing, China; [2] The State Key Laboratory of Hollow Fibre Membrane Materials and Processes, Tianjin Polytechnic University, Tianjin, China

年份：2013

卷号：5

期号：10

起止页码：402

外文期刊名：Journal of Chemical and Pharmaceutical Research

收录：EI(收录号：20134817024501)、Scopus(收录号：2-s2.0-84887950490)

语种：英文

外文关键词：Biological organs - Hydrolases - Metabolites - Optimization - Sugars

外文摘要：To increase the efficiency of biotransforming saponins in Dioscorea zingiberensis by Trichoderma reesei, four saponin hydrolases (E1, E2, E3 and E4) were purified and characterized. The molecular weights of E1, E2, E3 and E4 determined by SDS-PAGE were 50, 83, 67 and 46 kDa, respectively. E2 and E3 retained high activities at 20-60 °C. Based on HPLC-MS analysis, the substrate preferences of E1, E2, E3 and E4 were carefully studied. Both E2 and E3 could cleave C-26 glycoside, C-3 terminal glycoside, C-3 terminal rhamnoside and C-3 glycoside attached to aglycone in saponins. Four parameters (pH, T, Ca2+ and K+) which influenced the performances of E2 and E3 significantly were optimized for high biotransformation efficiency. After optimization, saponins in Dioscorea zingiberensis were efficiently hydrolyzed by the crude enzyme from Trichoderma reesei, with a diosgenin yield of 94.9%.