文献类型：期刊文献

中文题名：nNOS、Pax3和Cx43蛋白在人胚胎早期脊髓中的表达及意义

英文题名：EXPRESSION AND SIGNIFICANCE OF nNOS,PAX3 AND CX43 PROTEINS IN EARLY DEVELOPING SPINAL CORD OF HUMAN EMBRYOS

作者：刘学红[1];张泳[2];张金萍[1]

机构：[1]绍兴文理学院医学院组织学与胚胎学教研室;[2]绍兴文理学院医学院基础医学实验中心

年份：2008

卷号：39

期号：4

起止页码：594

中文期刊名：解剖学报

外文期刊名：Acta Anatomica Sinica

收录：CSTPCD、、北大核心2004、Scopus、CSCD2011_2012、北大核心、CSCD

语种：中文

中文关键词：脊髓;神经元型一氧化氮合酶;连接蛋白43;转录调控因子成对盒3蛋白;免疫组织化学;人胚胎

外文关键词：Spinal cord; Neuronal nitric oxide synthase; Connexin 43; Paired box 3; lmmunohistochemistry; Human embryo

中文摘要：目的探讨神经元型一氧化氮合酶（nNOS）、转录调控因子成对盒3（Pax3）和连接蛋白43（Cx43）在人胚胎发育早期脊髓中的分布规律及其表达意义。方法应用免疫组织化学SABC法,检测第5～16周人胚胎脊髓前角中nNOS、Pax3和Cx43蛋白的表达情况。结果在第5～16周,nNOS和Pax3蛋白在人胚胎脊髓前角细胞中由弱阳性逐渐变为阳性表达;在第5～12周,Cx43蛋白在人胚胎脊髓前角细胞中均呈阴性表达;第13～16周,Cx43蛋白在人胚胎脊髓前角细胞中呈阳性表达。结论nNOS、Pax3和Cx43蛋白与人胚胎脊髓的生长发育关系密切。

外文摘要：Objective To explore the rule of distribution and the expression significance of neuronal nitric oxide synthase （ nNOS）, paired box 3（Pax3） and connexin 43（Cx43） proteins in the early developing spinal cord of human embryos. Methods During the 5th to 16th weeks of human gestation, the expression productions of nNOS, Pax3 and Cx43 proteins were investigated in the anterior horn of spinal cord using the immunohistochemical methods. Results During the 5th to 16th weeks of human gestation, the immunohistochemical staining intensities of both nNOS and Pax3 in the anterior horn neurons of spinal cord increased from weakly to strongly positive staining. During the 5th to 12th weeks of human gestation, the immunohistochemical negative staining of Cx43 protein was observed in the anterior horn neurons of spinal cord, while the positive staining was observed from the 13th to 16th weeks. Conclusion nNOS, Pax3 and Cx43 proteins are related to the growth and development of spinal cord in human embryos.