文献类型：期刊文献

英文题名：Tanshinone IIA inhibits proliferation and activates apoptosis in C4-1 cervical carcinoma cells in vitro

作者：Li, Mingcheng[1]; Wang, Gang[1]; Zhang, Ruowen[2]; Duan, Siqi[1]; Chen, Jiayu[3]

机构：[1] Department of Clinical Laboratory, School of Laboratory Medicine, Beihua University, Jilin, China; [2] Department of Medicine, School of Medicine, Beihua University, Jilin, China; [3] Deptartment of Clinical Laboratory, School of Medicine, Shaoxing University, Shaoxing, Zhejing, China

年份：2019

卷号：33

期号：1

起止页码：1599

外文期刊名：Biotechnology and Biotechnological Equipment

收录：EI(收录号：20195207929105)、Scopus(收录号：2-s2.0-85076897447)

语种：英文

外文关键词：Cell proliferation - Electrophoresis - Flow cytometry - Proteins

外文摘要：Tanshinone IIA (Tan IIA) is a natural product that has been identified to have anti-proliferative properties against cervical cancer. The study was designed to investigate the anti-tumor effects of Tan IIA at different concentrations on the proliferation and apoptosis of human cervical carcinoma C4-1 cells. Human cervical carcinoma C4-1 cells were treated with different doses of Tan IIA, and MTT assay was performed to determine effects on cell viability. Flow cytometry was used to detect apoptosis and Western blot analysis was carried out to determine alterations in the expression of key proteins. Tan IIA treatment (0.5, 1.0, 2.0, and 5.0 mg/mL) significantly inhibited the growth of C4-1 cells in a time- and dose-dependent manner. Significant differences were observed in the growth inhibition rate between Tan IIA group and the control group (0.5-1.0 mg/L Group: p 0.05]). Western blot assays indicated that the Tan IIA decreased Bcl-2, HPV 16 E6 and E7 protein levels, but elevated Bax and cleaved-Caspase-3 expression in cells. Tan IIA may inhibit the proliferation and induce the apoptosis of C4-1 cells in a concentration-dependent manner. ? 2019, ? 2019 The Author(s). Published by Taylor & Francis Group on behalf of the Academy of Forensic Science.