文献类型：期刊文献

英文题名：Proteomics and bioinformatics analysis of cardiovascular related proteins in offspring exposed to gestational diabetes mellitus

作者：Pan, Hai-Tao[1,2,3];Xiong, Yi-Meng[2];Zhu, Hong-Dan[1,3];Shi, Xiao-Liang[1,3];Yu, Bin[1,3];Ding, Hai-Gang[1,3];Xu, Ren-Jie[1,3];Ding, Jin-Long[1,3];Zhang, Tao[1,3];Zhang, Juan[1,3]

机构：[1]Shaoxing Matern & Child Hlth Care Hosp, Shaoxing, Peoples R China;[2]Shanghai Jiao Tong Univ, Int Peace Matern & Child Hlth Hosp, Sch Med, Shanghai, Peoples R China;[3]Shaoxing Univ, Obstet & Gynecol Hosp, Shaoxing, Peoples R China

年份：2022

卷号：9

外文期刊名：FRONTIERS IN CARDIOVASCULAR MEDICINE

收录：SCI-EXPANDED(收录号：WOS:000874242100001)、、Scopus(收录号：2-s2.0-85140218144)、WOS

基金：This work was supported by the National Natural Science Foundation of China (82071729), the China Postdoctoral Science Foundation (2020M681336), the Science Technology Department of Zhejiang Province, China (LGD20H040001, LGF21H040003, and LGF21H040004), the Health Commission of Zhejiang Province, China (2022RC277, 2022KY412, 2022KY413, and 2021KY375), and the Science Technology Department of Shaoxing, China (2020A13034 and 2020A13035).

语种：英文

外文关键词：Gamete and Embryo-Fetal Origins of Adult Diseases; gestational diabetes mellitus (GDM); offspring; umbilical vessel; iTRAQ

外文摘要：IntroductionPrevious studies have demonstrated that exposed to the initial suboptimal intrauterine environment of gestational diabetes mellitus (GDM) may increase risk of cardiovascular disease in adulthood. MethodsIn order to investigate the underlying mechanisms involved in the increased risk of cardiovascular diseases (CVDs) in the offspring of GDM, we applied a high-throughput proteomics approach to compare the proteomic expression profile of human umbilical vessels of normal and GDM offspring. ResultsA total of significantly different 100 proteins were identified in umbilical vessels from GDM group compared with normal controls, among which 31 proteins were up-regulated, while 69 proteins were down-regulated. Differentially expressed proteins (DEPs) are validated using Western blotting analysis. The analysis of these differently expressed proteins (DEPs) related diseases and functions results, performed by Ingenuity Pathway Analysis (IPA) software. Based on "Diseases and Disorders" analysis, 17 proteins (ACTA2, ADAR, CBFB, DDAH1, FBN1, FGA, FGB, FGG, GLS, GSTM1, HBB, PGM3, PPP1R13L, S100A8, SLC12A4, TPP2, VCAN) were described to be associated with CVD, especially in Anemia, Thrombus and Myocardial infarction. Functional analysis indicated that DEPs involved in many cardiovascular functions, especially in "vasoconstriction of blood vessel" (related DEPs: ACTA2, DDAH1, FBN1, FGA, FGB, and FGG). Upstream regulator analyses of DEPs identifies STAT3 as inhibitor of ACTA2, FGA, FGB, and FGG. ConclusionThe results of this study indicate that intrauterine hyperglycemia is associated with an elevated risk of cardiovascular risk in the offspring.