文献类型：期刊文献

英文题名：Novel evidence indicates the presence and replication of hepatitis B virus in breast cancer tissue

作者：Qin, Bo[1,2];Zhao, Kaitao[2];Wei, Jianguo[1];Wang, Xin[1];Xu, Minjun[1];Lang, Juan[1];Sun, Honggang[1];Jin, Ketao[1]

机构：[1]Shaoxing Univ, Affiliated Hosp 1, Shaoxing Peoples Hosp, 568 Zhongxing North Rd, Shaoxing 312000, Zhejiang, Peoples R China;[2]Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China

年份：2020

卷号：43

期号：1

起止页码：296

外文期刊名：ONCOLOGY REPORTS

收录：SCI-EXPANDED(收录号：WOS:000505696300025)、、Scopus(收录号：2-s2.0-85076501385)、WOS

基金：This work was supported by the National Natural Science Foundation of China (grant no. 81772537), Foundation of Zhejiang Province Medical Health (grant no. 2020357743), Foundation of Shaoxing Municipal Commission of Health and Family Planning (CN), Zhejiang Province of China (grant no. 2017QN001), and Shaoxing Municipal Science and Technology Commission, Zhejiang Province of China (grant no. 2017B70022).

语种：英文

外文关键词：hepatitis B virus; sodium taurocholate cotransporting polypeptide; breast cancer; liver tropism

外文摘要：As a member of the liver tropic virus family, hepatitis B virus (HBV) was thought to only infect and replicate within the liver. Sodium taurocholate co-transporting polypeptide (NTCP) has been identified as a functional cellular receptor and a major determinant of liver tropism and HBV entry level species specificity. In the present study, the Oncomine database was used to explore differences in NTCP expression among cancerous and normal tissues. The results revealed that NTCP was highly expressed in breast cancer (BC), which was subsequently verified in clinical samples. Furthermore, in the BC tissue of patients with chronic HBV, HBV antigens, viral DNA/RNA and specific viral particles were detected via immunohistochemistry, ELISA, western blotting, reverse transcription-quantitative PCR and electron microscopy. Different HBV biomarkers and Dane particles were detected in BC. Furthermore, high levels of HBV-specific RNAs, the characteristic signals of HBV replication, were also detected, indicating that HBV infects BC tissue by binding to NTCP and replicating within. Based on the data of the present study, BC tissue may represent a second location of HBV infection and replication in addition to the liver.