文献类型：期刊文献

英文题名：Modification of the second PEP4-allele enhances citric acid stress tolerance during cultivation of an industrial rice wine yeast strain with one PEP4-allele disrupted

作者：Zhang, Hongbo[1]; Shao, Feifei[1]; Cong, Jianhang[1]; Huang, Yan[1]; Chen, Minfang[1]; He, Wenxia[1]; Zhang, Tao[1]; Liu, Luyi[1]; Yao, Mengzhou[1]; Gwabin, Hannatu[1]; Lin, Yingjiang[1]

机构：[1] College of Life Sciences, Shaoxing University, 900 Chengnandadao Road, Shaoxing, 312000, China

年份：2021

卷号：152

外文期刊名：LWT

收录：EI(收录号：20213310784990)、Scopus(收录号：2-s2.0-85112487774)

语种：英文

外文关键词：Genes - Wine - Cultivation - Citric acid

外文摘要：To investigate the effect of PEP4 alleles on cellular tolerance of rice wine Saccharomyces cerevisiae against citric acid stress, one PEP4-allele disrupted strain and one PEP4-allele modified along with one PEP4-allele disrupted rice wine Saccharomyces cerevisiae strains were constructed. The PEP4-allele disruption and modification significantly decreased intracellular PrA activity, and a dose-response relationship was found between intracellular Proteinase A activity and number of intact PEP4-alleles. Under cultivation conditions whether with sufficiency or starvation of amino acid nitrogen source, the second PEP4-allele modification was all found beneficial for cellular citric acid stress tolerance of the industrial Saccharomyces cerevisiae strain SWY85. These results allude to PEP4-allele having a physiological role in the metabolic regulations correlated to citric acid tolerance in yeast cell. ? 2021 Elsevier Ltd